Reexamination of Methods for Determination of Chlorophyll in Pure Chlorophyll Solution and Mixture of Chlorophyll and Pheophytin

Abstract

Various quantitative analytical methods of chlorophyll and pheophytin have been reported. But any study has not been reported comparing them with each other. So we tried to reexamine methods of chlorophyll determination using purified chlorophyll from spinach leaves. In spite of different various methods, quantity value of chlorophyll was almost consistent. Determination of chlorophyll in a mixture solution of chlorophyll and pheophytin was very difficult, because the absorption spectra of chlorophyll and pheophytin were very similar. In such a case, most of methods produced error of quantity value of chlorophyll. But using the method proposed by DIETRICH, that was determination of conversion of chlorophyll to pheophytin, good results were obtained.