Identification of Important Rice Cultivars in Akita Prefecture with One Grain by STS-PCR Method

Abstract

The PCR (polymerase chain reaction) analysis improved from RAPD (random amplified polymorphic DNA) method was developed to identify with one grain rice samples. Five polymorphic RAPD markers discriminating 8 cultivars were cloned for DNA sequencing. Five sets of STS (sequence tagged-site) primers were designed based on the DNA sequences, and generated single bands identical to each discrimination markers in RAPD method. These results indicated that 9 important rice cultivars in Akita prefecture including Akitakomachi were clearly identified by band patterns of PCR. The STS-PCR analysis comparing with RAPD method facilitated simple and rapid procedure due to the single amplified band patterns.