Abstract
Gly m Bd 28K, one of the major soybean allergens, was analyzed in six leading varieties in Japan by immunoblot analysis using monoclonal antibody specific to Gly m Bd 28K. Among these, five varieties, which account for nearly half of the total soybean acreage in Japan lacked. Gly m Bd 28K. Since each of the five soybeans belongs to much different maturity groups, a gene or genes of lacking Gly m Bd 28K may exist commonly in Japanese domestic varieties. Fukuyutaka-QT2 line, which has been bred to lack α-, α' and β-subunits of β-conglycinin, lacks Gly m Bd 28K, same as its recurrent parent Fukuyutaka. Both Gly m Bd 28K and α-subunit of β-conglycinin have secondarily highest reactivity with the sera of soybean-allergic patients, and β-subunit of β-conglycinin is also one of the major soybean allergens which reacts with 10% of the sera. Therefore, Fukuyutaka-QT2 line would be a promising source for developing a less allergic soybean variety.
