Abstract
Enzymatic synthesis of aspartame precursor, N-(benzyloxycarbonyl)-L-aspartyl-L-phenylalanine methyl ester (ZAPM) was successfully performed using molten N-(benzyloxycarbonyl)-Laspartic acid (ZA) and L-phenylalanine methyl ester (PM) mixtures in the presence of salt hydrate (Na2HPO4·12H2O, NaCO3·10H2O) without any liquid addition. The mixture composed of ZA, PM, salt hydrate and enzyme could be preserved under the dehydrate temperature of salt without ZAPM synthesis during one year, and available for ZAPM synthesis by control the temperature up to the dehydrate temperature on occasion. This storable mixture is considerable as a useful master powder for ZAPM synthesis.
