Evaluation of Antioxidant Effect by Capillary Electrophoresis Assay

Abstract

We had tried to measure antioxidant effect of flavonoids on the oxidation of LDL using capillary electrophoresis. When LDL was oxidized in the presence of Cu²⁺, the mobility time of LDL was rightly shifted on the chromatogram with increased oxidation time. The elongation of the mobility time was inhibited by addition of BHT and ascorbic acid. When inhibitory effect of flavonoids on the LDL oxidation was examined, the oxidation of LDL was prevented in proportion to the number of OH group in the B ring of flavonoids. The capillary electrophoresis method is useful for evaluation of antioxidant effect of flavonoids on Cu²⁺ -induced LDL oxidation.