Inhibition of Intracellular Glucose Metabolism by 2-deoxyglucose Suppresses Proliferation and Differentiation of Mouse Osteoblast-like Cells

抄録

 Purpose: Glucose metabolism plays a critical role in osteoblast differentiation and influences vital processes involved in bone formation and remodeling. This study explored the inhibitory effects of 2-deoxyglucose (2-DG) on glucose uptake by osteoblasts and its impact on cell differentiation.

 Methods: Based on the structural similarity between 2-DG and glucose, the effects of glucose metabolism on osteoblast differentiation were examined by competitive inhibition of glucose uptake. MC3T3-E1 (mouse osteoblast-like cells) were cultured in media containing 10% FBS supplemented with 2-DG to evaluate cell proliferation and osteogenic differentiation.

 Results: Inhibition of intracellular glucose metabolism by 2-DG inhibited MC3T3-E1 cell proliferation and mineralization. However, the gene expression of Runx2 mRNA was enhanced in the 2-DG group. Moreover, there was a negligible difference in the osteocalcin production.

 Conclusion: Decreased intracellular glucose metabolism inhibited osteoblast proliferation and differentiation.