Abstract
Extreme care must be taken of room temperature when staining peroxidase granules of leucocytes, since change of temperature exerts a certain influence upon the peroxidase staining. The optimal temperature of the staining lies between 28°C and 37°C, and both lower and higher temperatures than this inhibit the staining in various degrees. The peroxidase reaction becomes entirely negative at 140°C for 5minutes, however it is still po3itive at 130°C for 5 minutes. The reaction never becomes negative even at 0°C for 10 hours, although the peroxidase index used to express the intensity of the reaction numerically, decreases clearly. The brown and blue granules shown in the peroxidase reation do not differ essentially with each other judging from the results of the experiments on the effect of change in temperature.
