Okajimas Folia Anatomica Japonica

A-B-O Blood Groups of the Japanese: an Anthropological Study

Toshiyasu Hirokawa

1952 Volume 24 Issue 2 Pages 61-75_1
Published: 1952
Released on J-STAGE: September 24, 2012

DOIhttps://doi.org/10.2535/ofaj1936.24.2_61

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On the Mitochondria of Ovarian Egg-Cells of an Osseous Fish (Leiognathus argenteum)

Morio Ihnuma, Seiji Minamitani

1952 Volume 24 Issue 2 Pages 77-80_2
Published: 1952
Released on J-STAGE: September 24, 2012

DOIhttps://doi.org/10.2535/ofaj1936.24.2_77

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1. Mitochondria of ovarian egg-cells of Leiognathus argenteum were observed.
2. Selection of proper fixative solutions and staining procedures is necessary for each stage in the development of egg-cells.
3. In young egg-cells, mitochondria are granular. In the stage just before yolk formation, threadlike mitochondria appear and gradually increase in later stages till the stage of active yolk formation, when all mitochondria have threadlike shape. After the yolk formation is completed, these threadlike mitochondria disappear and all mitochondria present are granular ones.
4. In the stage just before yolk granules appear, mitochondria disappear from the portion of cytoplasm around the nucleus. When yolk granules have grown larger, mitochondria appear again in all parts of cytoplasm.

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On the Various Alums as the Peroxidase Stain of Blood Cells. The 4th Report of Histochemical Study of Peroxidase

Mamoru Sasaki

1952 Volume 24 Issue 2 Pages 81-87
Published: 1952
Released on J-STAGE: September 24, 2012

DOIhttps://doi.org/10.2535/ofaj1936.24.2_81

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The alum is one of double salts of sulfate and its aqueous solution is highly acid. Lithium alum and cobalt alum are useful in staining the peroxidase positive granules of leucocyte blue or bluish violet, while the other nine alums are unsuitable as far as the fixation and peroxidase staining of blood cells are concerned. Most of alums except cerium alum belong to the Salt I after Mitsui and Ikeda, in other words, their saturated aqueous solutions become inhibitor of this histochemical peroxidase reaction. Furthermore all of them lose their potency within a day when mixed with the benzidine-hydrogen-peroxide solution in a test-tube before the staining.

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Study on the Influence of Temperature Upon the Peroxidase Reaction of Blood Cells. The 5th Report of Histochemical Study of Peroxidase

Koichi Tsukamoto

1952 Volume 24 Issue 2 Pages 89-97
Published: 1952
Released on J-STAGE: September 24, 2012

DOIhttps://doi.org/10.2535/ofaj1936.24.2_89

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Extreme care must be taken of room temperature when staining peroxidase granules of leucocytes, since change of temperature exerts a certain influence upon the peroxidase staining. The optimal temperature of the staining lies between 28°C and 37°C, and both lower and higher temperatures than this inhibit the staining in various degrees. The peroxidase reaction becomes entirely negative at 140°C for 5minutes, however it is still po3itive at 130°C for 5 minutes. The reaction never becomes negative even at 0°C for 10 hours, although the peroxidase index used to express the intensity of the reaction numerically, decreases clearly. The brown and blue granules shown in the peroxidase reation do not differ essentially with each other judging from the results of the experiments on the effect of change in temperature.

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Effect of Streptomycin Upon the Peroxidase Staining of Blood Cells. The 6th Report of Histochemical Study of Peroxidase

Fusao Morita

1952 Volume 24 Issue 2 Pages 95-98
Published: 1952
Released on J-STAGE: September 24, 2012

DOIhttps://doi.org/10.2535/ofaj1936.24.2_95

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Aqueous solution of streptomycin made in U.S.A. or Japan exerts distinct effects upon the peroxidase staining. The concentration effective enough to show blue peroxidase positive granules in the myelogenous leucocytes, lies between 70 per cent (saturated) and 0.2 per cent. Remarkable difference between streptomycin made in U.S.A. and that in Japan was not seen. This characteristic potency of streptomycin probably originates from the salt contained in it which can form blue "Merichinoid" complex with benzidune.

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Comparison Among Various Peroxidase Staining Methods by Means of Pictures. The 7th Report of Histochemical Study of Peroxidase

Tadao Mitsui, Fusao Morita, Mamoru Sasaki, Koichi Tsukamoto

1952 Volume 24 Issue 2 Pages 99-101_1
Published: 1952
Released on J-STAGE: September 24, 2012

DOIhttps://doi.org/10.2535/ofaj1936.24.2_99

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On the Mitochondria, Ribonucleic Acid, Acid Phosphatase and Polysaccharide of Liver Cells of a Fish (Oryzias latipes) during Starvation

II. On Acid Phosphatase

Tomi Tsukuda

1952 Volume 24 Issue 2 Pages 103-106_1
Published: 1952
Released on J-STAGE: September 24, 2012

DOIhttps://doi.org/10.2535/ofaj1936.24.2_103

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1. Starvation experiments of ten days, twenty days and thirty days were carried out and the changes of acid phosphatase activity were observed on the liver of Oryzias latipes.
2. The activity of acid phosphatase is obviously intensified temporarily by starving for ten days and then dereases rapidly as the duration of starvation become longer.
3. In the final stage of starvation the enzyme activity remains positive only on the nucleolus.
This study was performed with the aid of The Subsidiary Fund for Science Promotion from Keio-Gijuku given to M. Ihnuma, the Assistant Professor of anatomy.

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Über die GröBe der Querschnittfläche des Haarbalges in den verschiedenen körperteilen bei einigen Menschenrassen.

Korezune Fujino

1952 Volume 24 Issue 2 Pages 107-135
Published: 1952
Released on J-STAGE: September 24, 2012

DOIhttps://doi.org/10.2535/ofaj1936.24.2_107

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