Essintial elements deficiency or heavy metal poisoning were observed clinically. Physiological functions of trace elements in organism have not been known well. The purpose of this study was to verify the physiological validity of using trace elements in colonic mucosa as a mesure of the cancer stage. Therefore, we analyzed various trace elemens in colonic mucosa. Fifteen colonic adenomas were chosen as subjects of the study. Ten cases of normal colonic mucosa were used as the control group.
The biopsy of colonic mucosa was performed with well informed concent using an electric endoscope EVIS-200HI (Olympus Co) . The sample was lyophilized at －80℃ immediatery. The feature of PIXE is its ability to analyze trace elements quantitatively without decomposing samples. Pd thin layer was deposited onto the sample on carbon adhesive tape uising metal ion sputtering equipment. The proton energy was 2.3MeV, 10nA. The beam spot area was 2×3mm, and the irradiation time was approximatery 10 minutes. Proton bombardment was carried out at National Institute of Radiological Sciences.
Calcium, zinc, copper, iron, were determined quantitativerly. Among trace elements, Zn, and Cu and among non-trace elements Ca, and Fe were highly significant among three groups (significant at the p＜0.1% level) . The concentration of Zn, Cu and Fe were higher in the adenoma group than in control group.
