A“bulb”subregion in the vacuolar membrane

Chieko Saito

doi:10.5685/plmorphol.15.60

Chieko Saito

著者情報

キーワード: Arabidopsis, cotyledon, green fluorescence protein, metal-contact type rapid freezing, membrane dynamics, vacuole

ジャーナルフリー

2003 年 15 巻 1 号 p. 60-67

DOI https://doi.org/10.5685/plmorphol.15.60

詳細

Published: 2003 Received: - Available on J-STAGE: 2010/06/28 Accepted: - Advance online publication: - Revised: -
訂正情報
訂正日: 2010/06/28 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2010/06/28 訂正理由: - 訂正箇所: キーワード情報訂正内容: Wrong : Arabidopsis, cotyledon, green fluorescence protein, metal-contact type rapid freezing, membrane dynamics, vacuole
Right : Arabidopsis, cotyledon, green fluorescence protein, metal-contact type rapid freezing, membrane dynamics, vacuole

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抄録

Complex, mobile spherical structures continuous with the vacuolar limiting membrane were identified in young cotyledons of Arabidopsis by expression of a vacuolar membrane marker(r-TIP-GFP)and by electron microscopy, and were named“bulbs”. These structures are cytoplasm wrapped by two layers of intricately folded membrane; both layers are continuous with the vacuolar limiting membrane. When visualized using r-TIP-GFP, the fluorescence intensity of bulbs is about3-fold higher than that of the vacuolar limiting membrane. However, bulbs were negative for GFP-AtRab75, another vacuolar marker. These results suggest that bulbs form a subregion in the continuous vacuolar membrane that concentrate some proteins and segregate others.

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