Intracellular positioning of nucleus and mitochondria with clumping of chloroplasts in the succulent CAM plant Kalanchoe blossfeldiana: an investigation using fluorescence microscopy

Ayumu Kondo; Keiko Shibata; Tatsuya Sakurai; Masahiro Tawata; Toru Funaguma

doi:10.5685/plmorphol.18.69

Ayumu Kondo, Keiko Shibata, Tatsuya Sakurai, Masahiro Tawata, Toru Funaguma

著者情報

キーワード: fluorescence microscopy, organelle interactions, three-dimensional analysis, water stress

ジャーナルフリー

2006 年 18 巻 1 号 p. 69-73

DOI https://doi.org/10.5685/plmorphol.18.69

詳細

Published: 2006 Received: - Available on J-STAGE: 2010/06/28 Accepted: - Advance online publication: - Revised: -
訂正情報
訂正日: 2010/06/28 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2010/06/28 訂正理由: - 訂正箇所: キーワード情報訂正内容: Wrong : Key words: fluorescence microscopy, organelle interactions, three-dimensional analysis, water stress.
Right : fluorescence microscopy, organelle interactions, three-dimensional analysis, water stress

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抄録

Summary: In leaves of some succulent crassulacean-acid-metabolism(CAM)plants, including Kalanchoe species, we previously discovered the phenomenon of chloroplast clumping. A combination of light and water stress induced chloroplasts to form spherical bodies within mesophyll cells. In this study we focused on the positioning of nuclei and mitochondria with clumping of chloroplasts in mesophyll cells in leaves of the succulent CAM plant Kalanchoe blossfeldiana using fluorescence microscopy. Under both well-watered and water-stressed conditions, there were several mitochondria around each chloroplast. When subjected to water-stress, chloroplasts gathered around a nucleus and formed a clumped chloroplasts. Three-dimensional analysis of the clump showed that the nucleus was localized within the clump. Our findings suggest that the chloroplast movement in succulent CAM plants is closely connected with the nucleus. We discuss the significance of the organelle interactions in the leaves of succulent CAM plant under drought conditions.

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