Structure of the organellar nuclei in Euglena revealed by immunoelectron microscopy

Katsumi Ueda

doi:10.5685/plmorphol.8.37

免疫電顕法によるユーグレナのオルガネラ核の構造

植田勝巳

著者情報

ジャーナルフリー

1996 年 8 巻 1 号 p. 37-44

DOI https://doi.org/10.5685/plmorphol.8.37

詳細

発行日: 1996 年受付日: - J-STAGE公開日: 2010/06/28 受理日: - 早期公開日: - 改訂日: -
訂正情報
訂正日: 2010/06/28 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2010/06/28 訂正理由: - 訂正箇所: キーワード情報訂正内容: Wrong : Key words: anti-DNA antibody, Euglena, immunoelectron microscopy, nucleoid, organellar nuclei
Right : anti-DNA antibody, Euglena, immunoelectron microscopy, nucleoid, organellar nuclei

訂正日: 2010/06/28 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E

Right : Alegre C, Subirana JA(1989)The diameter of chromatin fibres depends on linker length. Chromosoma 98: 77-80
Bak AL, Zeuthen J, Crick FHC(1977)Higher-order structure of human mitotic chromosomes. Proc Natl Acad Sci 74: 1595-1599
Bahr GF(1970)Human chromosome fibers. Considerations of DNA-protein packing and of looping patterns. Exp cell Res 62: 39-49
Carpenter BG, Baldwin JP, Bradbury M, Ibel K(1976)Organization of subunits in chromatin. Nucleic Acid Res 3: 1739-1746
Coleman AW(1978)Visualization of chloroplast DNA with two fluorochromes. Exp. Cell Res. 114: 95-100
Earnshaw WC, Laemmli UK(1983)Architecture of metaphase chromosomes and chromosome scaffolds. J Cell Biol 96: 84-93
Finch JT, Klug A(1976)Solenoid model for superstructure in chromatin. Proc Nat Acad Sci 73: 1897-1901
Hayashi-Isimaru Y, ueda K, Nonaka M(1993)Detection of DNA in the nucleoids of chloroplasts and mitochondria in Euglena gracilis by immunoelectron microscopy. J Cell Sci 105: 1159-1164
Kellenberger E, Ryter A, Sechaud J(1958)Electron microscope study of DNA containing plasms. II Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J. Biophys. Biochem. Cytol. 4: 671-678
Kuroiwa T(1982)Mitochondrial nuclei. Int. Rev. Cytol. 75: 1-59
Leedale, GF(1968)The nucleus in Euglena. in: Buetow DE(ed)The Biology of Euglena Vol 1. Academic Press, New York, pp185-243
Marsden MPF, Laemmli UK(1979)Metaphase chromosome structure: Evidence for a radial loop model. Cell 17: 849-858
Moyne G, Bertaux 0, Puvion E(1975)The nucleus of Euglena I. An ultracytochemical study of the nucleic acids and nucleoproteins of synchronized Euglena gracilis Z. J Ultrastr Res 52: 362-376
Nass MMK, Nass S(1963)Intramitochondrial fibers with DNA characteristics. I. Fixation and electron staining reactions. J. Cell Biol. 19: 613-629
Okada TA. Comings DE(1979)Higher order structure of chromosomes. Chromosoma 72: 1-14
Olins AL, Olins DE(1974)Spheroid chromatin units(v body). Science 183: 330-332
Paulson JR(1989)Scaffold morphology in histone-depleted HeLa metaphase chromosomes. Chromosoma 97: 289-295
Ris H(1966)Fine structure of chromosomes. Proc R Soc Lond B 164: 246-257
Ueda K, Hayashi-Ishimaru Y(1996)Localization of DNA in the condensed interphase chromosomes of Euglena. Chromosoma 104: 380-385.
Yokomura E(1967)An electron microscopic study of DNA-like fibrils in chloroplasts. Cytologia 32: 361-377

抄録

The localization of DNA in the condensed interphase chromosomes and of DNA in the nucleoids of chloroplasts and mitochondria in cells of Euglena was studied by immunoelectron microscopy. Small colloidal gold(5-6nm in diameter)was conjugated with anti-mouse IgM antibodies. Deposits of gold particles on the interphase chromosomes that coincided with the localization of DNA followed threads that corresponded to the chromatin fibers. The threads were50-80nm in diameter. The localization of gold deposits on chromosomes that had been sectioned in various directions suggested that the chromatin fibers coiled around the surface of chromosomes, with a wide central axial region of the chromosomes remaining free of DNA. The structure of the nucleoids in chloroplasts and in mitochondria was very different from those previously observed by conventional electron microscopy. Nucleoids in chloroplasts appeared to be composed of twisted threads50-70nm in diameter. Mostnucleoids in mitochondria were spherical or ovoid, 70-130nm in diameter. Nucleoids both in chloroplasts and in mitochondria contained cores with which DNA threads were in tight contact.

責任著者(Corresponding author)

訂正情報

J-STAGEへの登録はこちら（無料）