抄録
Expressional regulation of estrogen-responsive genes in adult female rats. Wada-Kiyama Y.1), Rho J.-y.2), Kiyama R.3), Sakuma Y.1) 1) Nippon Med. Sch., Tokyo, Japan. 2) Korea Insti. Biosci. Biotech., Daejeon, Korea. 3) RCG, AIST, Ibaraki, Japan. In rats, estrogen plays critical roles in sexual differentiation of the brain and regulation of the sexual behavior. To examine the regulatory mechanism of estrogen in rats, we prepared 8-weeks ovariectomized rats and treated with or without estrogen for two weeks. mRNA was obtained from the hypothalamus, uterus, liver, kidney and skeletal muscle and analyzed by Northern blotting and/or RT-PCR. Neuronal system-related genes (Amphiregulin, Neuropeptide Y-Y1 receptor, Bassoon, N-Cadherin) and estrogen-susceptible cancer-related gene (C-terminal binding protein interacting protein) were selected based on the results of a cDNA microarray analysis which profiled estrogen-responsive genes in MCF-7 cell line. In the hypothalamus, all except the Amphiregulin gene were down-regulated. In other tissues, the expression showed marked differences. We then analyzed the time course of the estrogen-responsiveness of these genes, finding changes in expression patterns especially in the skeletal muscle. Our results show that the estrogen-responsive genes, which were demonstrated simply as either up- or down-regulation by estrogen in MCF-7, exhibit tissue and temporal-specific expression patterns in adult female rats. [Jpn J Physiol 54 Suppl:S219 (2004)]
