日本生理学会大会発表要旨集
日本生理学会大会発表要旨集
セッションID: 2O-E-13
会議情報

細胞間隙を通って内皮下へ浸潤する単球近傍への内皮細胞PECAM-1の局所集積 –分子イメージングによる3次元動態解析–
*橋本 謙片岡 則之中村 恵美萩原 喜美子辻岡 克彦梶谷 文彦
著者情報
会議録・要旨集 フリー

詳細
抄録
To shed light on the specific role of the transmembrane protein PECAM-1 in monocyte diapedesis, PECAM-1-GFP fusion vector was constructed and transfected into human umbilical vein endothelial cells (HUVECs). Since GFP was fused to the C-terminus (intracellular side) of PECAM-1, interactions of the PECAM-1 extracellular domain with monocytes can be kept intact. Three-dimensional-live-cell molecular imaging revealed that endothelial PECAM-1 was dynamically recruited to surround the transmigrating monocyte locally during paracellular diapedesis. This recruitment was not observed during transcellular diapedesis, which was only 5% of total diapedesis events. Three-dimensional image analysis showed a <1.5–1.8 fold increase of local PECAM-1 fluorescent signals near transmigrating monocytes within 20 min of paracellular events. Flow cytometric analysis also indicated a <1.4-fold increase of overall PECAM-1 signals from endothelial cells in 50 min of monocyte coincubation. The recruitment was not seen in the local region which was not associated with diapedesis events, which excludes the involvement of soluble factors secreted by monocytes. Blocking of neither monocyte adhesion nor transmigration induced the recruitment. These data indicate that local transmigration events, but not adhesion, or monocyte soluble factors, could induce the dynamic recruitment of endothelial PECAM-1 to transmigrating monocytes during paracellular diapedesis. [J Physiol Sci. 2008;58 Suppl:S61]
著者関連情報
© 2008 日本生理学会
前の記事 次の記事
feedback
Top