抄録
The fundamental study for the size distribution of red cells and platelets was performed by using the electronic cell counter- Coulter Counter, Model ZBI and Channelzyer. Blood counts by Coulter ZBI coincided well with those by the conventional hemocytometer. Edit switch was kept ON in order to eliminate irregular size distribution curves due to long impulses from cells passing through simultaneously into the orifice tube. No significant difference of size distribution curves of red cells was observed by the use of various anticoagulants such as ACD, citric acid, EDTA, heparin and Anglot ET or by the time duration after getting blood samples. However, significant decrease of platelet counts was observed 2 hours after the collection of samples. No significant difference was seen in platelet size distribution. The study was done just after the collection of samples.
The mean cell volume (MCV) from Channelyzer was compared to MCV calculated from red cell counts and hematocrit value. There was significant positive correlation between them (r=0.734, p<0.01) and MCV from Channelyzer was a little bit smaller than that from hematocrit. Average platelet diameter and volume in normal subjects were 3.41 μ±0.35 and 7.31 μ3±0.73, respectively, which coincided well with values reported by the other investigaters.
The application of Channelyzer to the clinical use was presented in the change of red cell distribution curves during the treatment of iron deficiency anemia.
