Sulfur dioxide in dried Cortinellus Shiitakewas detected by the method of starch potassium iodate paper and malachite green's reactions. But sulfur dioxide was not detected in natural Cortinellus Shiitake. The amount of sulfur dioxide determined by Monnier-Williams's method was 0.01-0.13g/kg in dried Cortinellus Shiitake.
When dried Cortinellus Shiitake was wetted in water, the amount of sulfur dioxide increased during four hours. After wetting six hours later, sulfur dioxide level became constant. The amount was not influenced by drying method, drying time, nor by the place of production and the kind of Cortinellus Shiitake. When lenthionine was added to dried Cortinellus Shiitake, increase of the amount of sulfur dioxide was not observed. In view of these facts, it was supposed that, when dried Cortinellus Shiitake was wetted in water and was boiled with 25% phosphoric acid, sulfur compounds of Cortinellus Shiitake are decomposed to produce a substance which is positive to the sulfur dioxide reaction.