Abstract
A solution containing saccharin, cyclohexane sulfamic acid and dulcin which have been obtained from a preparation or food is allowed to stand in a centrifugal tube for 10 minutes with Amberlite CG 400 Type I (Cl-form) with shaking sometimes and centrifuged. The elution of saccharin and cyclohexane sulfamic acid from the resins is carried out by means of a mixture of 10% oxalic acid and alcohol. The eluate is neutralized with aqueous ammonia and applied to the paper chromatography. Dulcin is obtained by extracting the supernatant with etbylacetate.
Saccharin, cyclohexane sulfamic acid and dulcin are detected by the ascending paper chromatography with iso-amyl alcohol-pyridine-water, or n-butanol-aqueous ammonia. In order to identify these artificial sweeteners when they are accompanied with some food preservatives, such as salicylic acid, benzoic acid, sorbic acid, etc., the former solvent is an excellent one as a developer.
