The occurrenc of gobies (Family Gobiidae) in the southern region of Urado Estuary in Kochi City was studied from April 2004 to January 2005. A total of 27 species belonging to 22 genera were recorded. Acanthogobius lactipes (endangered species of Kochi Prefecture), Apocryptodon punctatus (vulnerable species), two near endangered species Drombus sp. and Pseudogobius masago, and Bathygobius hongkongensis were first obtained from Urado Estuary. Specimens of Psammogobius biocellatus and Oxyurichthys ophthalmonema represented the second record for each species in Kochi Prefecture. The occurrences of the following four endangered species of Kochi Prefecture were also confirmed: Callogobius tanegasimae, Eutaeniichthys gilli, Periophthalmus modestus and Taenioides cirratus. These facts strongly suggested that Urado Estuary is one of the important brackish water environments in Kochi Prefecture.