(1) The permeability of the cell of the laver (Porphyra tenera) to some nitrogenous compounds such as potassium nitrate, ammonium nitrate, ammonium sulfate and urea was investigated by the method of plasmolysis critical concentration.
The permeable coefficients estimated by the TRÖNDL's formula were 0.50 in the potassium nitrate, 0.62 in the ammonium nitrate, 0.64 in the ammonium sulfate and 0.58 in the urea.
It was found that the permeability to the urea was not so great, but the penetration of this was ascertained by the cytochemical detection as minute granules within the cytoplasm.
(2) After some mechanically injurious operations to the cells, such as mere pricks with a needle, if a piece of the injured Porphyra-frond was transfered from the higher osmotic medium to the lower one, some injured cells become into amoeboid shape, and the pseudo-podia like swellings attack the adjointing cells one after another, making a “plasmodium-like” radial figure. These figures were recognized with the naked eye as green spots on the same feature as the “perforating disease” previously reported by SUTO & UMEBAYASHI as a parasitic disease of the laver.