組織中のクロルテトラサイクリンの簡易cylinder plate法

富山 哲夫; 小林 邦男; 米 康夫

doi:10.2331/suisan.24.937

富山哲夫, 小林邦男, 米康夫

著者情報

ジャーナルフリー

1959 年 24 巻 11 号 p. 937-942

DOI https://doi.org/10.2331/suisan.24.937

詳細

発行日: 1959 年受付日: - J-STAGE公開日: 2008/02/29 受理日: 1958/12/08 早期公開日: - 改訂日: -
訂正情報
訂正日: 2008/02/29 訂正理由: - 訂正箇所: 論文抄録訂正内容: Wrong : The regular bioassay method for determination of chlortetracycline (CTC) residue in tissues^{1, 2, 3, 4)} presents a difficulty when dealt with many samples for the analysis. Experiences have revealed that such procedures are very tedious as washing and drying of many Petri dishes, the preparation of assay medium in each dish and the standard curve for each set of determination.
Attempt has been made, therefore, to simplify the method without sacrificing its accuracy. It was ascertained that routine procedures could be greatly simplified by substituting the Petri dish with glass plate as was reported by GRADY and WILLIAMS^1a). It was found that the slope of inhibitory zone size on logarithm of CTC concentration remained constant irrespective of difference in plate and date of the determination. Therefore, the preparation of the standard curve at each determination can be omitted by employing the linear regression line instead when it is calibrated for the difference in zone size for 0.01mcg/cc between the regression line and a sample test plate.
Right : The regular bioassay method for determination of chlortetracycline (CTC) residue in tissues^{1, 2, 3, 4)} presents a difficulty when dealt with many samples for the analysis. Experiences have revealed that such procedures are very tedious as washing and drying of many Petri dishes, the preparation of assay medium in each dish and the standard curve for each set of determination.
Attempt has been made, therefore, to simplify the method without sacrificing its accuracy. It was ascertained that routine procedures could be greatly simplified by substituting the Petri dish with glass plate as was reported by GRADY and WILLIAMS^1a). It was found that the slope of inhibitory zone size on logarithm of CTC concentration remained constant irrespective of difference in plate and date of the determination. Therefore, the preparation of the standard curve at each determination can be omitted by employing the linear regression line instead when it is calibrated for the difference in zone size for 0.01mcg/cc between the regression line and a sample test plate.

訂正日: 2008/02/29 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

The regular bioassay method for determination of chlortetracycline (CTC) residue in tissues^{1, 2, 3, 4)} presents a difficulty when dealt with many samples for the analysis. Experiences have revealed that such procedures are very tedious as washing and drying of many Petri dishes, the preparation of assay medium in each dish and the standard curve for each set of determination.
Attempt has been made, therefore, to simplify the method without sacrificing its accuracy. It was ascertained that routine procedures could be greatly simplified by substituting the Petri dish with glass plate as was reported by GRADY and WILLIAMS^1a). It was found that the slope of inhibitory zone size on logarithm of CTC concentration remained constant irrespective of difference in plate and date of the determination. Therefore, the preparation of the standard curve at each determination can be omitted by employing the linear regression line instead when it is calibrated for the difference in zone size for 0.01mcg/cc between the regression line and a sample test plate.

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