Crystalline specimens of tuna, skipjack, and blue-white dolphin Mb's were prepared and analyzed for N-terminal structure. No N-terminal amino acid was detected by the DNP method in tuna and skipjack Mb's, whereas 1N-terminal glycyl residue per molecule was detected in blue-white dolphin Mb. One acetyl group per molecule was determined in each Mb by gas chromatographic and colorimetric methods. Based on those results, it may be concluded that both fish Mb's have an acetylated N-terminal amino acid residue, while blue-white dolphin Mb has a glycyl residue as N-terminus and an acetyl group at the other position of the molecule.