Hemoglobins (Hb) of five subspecies of “funa”, Japanese crucian carp, i.e., “kinbuna”, “ginbuna”, “gengorobuna”, “nigorobuna”, and “nagabuna”, were analyzed by the starch gel electrophoretic method. Results obtained are summarized as follows: (1) More than two Hb patterns were presented by those subspecies except “nagabuna”. Through all the patterns observed, most components showed common migration velocities and the slowest component was almost always predominant. (2) As far as the Hb patterns are concerned, “kinbuna” and “ginbuna” seem to be very closely related to each other. So is the case between “nigorobuna” and “nagabuna”. On the other hand, “gengorobuna” appears to be somewhat distantly related to those four subspecies.