In the present study, the sterol compositions of the lobster, Panulirus japonica, prawn, Penaeus japonicus, crab, Portunus trituberculatus, Amphipoda, Caprella sp., Mysidacea, Neomysis intermedia, and 3 species of mantis crabs, Gonodactylus falcatus, Gonodactylus chiragra, and Odontodactylus scyllarus, were investigated by using gas-chromatography. Cholesterol was the major sterol (73-100%) in these animals. In the case of prawn, Am-phipoda, and Mysidacea, the other sterols were contained as a consituents in addition to cholesterol. 22-Dehydrocholesterol was present in the prawn (3%), Amphipoda (4%), and Mysidacea (3%). The presence of 24-methylenecholesterol was also demonstrated in the above 3 crustaceans. Brassicasterol and desmosterol were found in the Amphipoda (22%) and Mysidacea (8%), respectively.