海洋細菌の無機塩要求性に関する生理学的研究-II

コハク酸およびフマール酸酸化におよぼす無機塩の影響

抄録

Inorganic salt requirements of M-type (marine type), MH-type (marine halophilic type), TH-type (terrestrial halophilic type) and T-type (terrestrial type) bacteria for succinic acid and fumaric acid oxidations were investigated with the Warburg respirometer. A typical strain of each type required the following salts for the optimum oxidation of succinic acid at 25°C: M-type, NaCl 220mM+KCl 10mM+CaCl₂ 5mM+MgCl₂10mM+MgSO₄ 10mM; MH-type, NaCl 370mM+KCl 10mM+MgCl₂ 10mM; TH-tpye, NaCl 180mM+KCl 10mM+MgSO₄ 5mM;T-type, KCl 5mM. It was suggested that M-, MH-, TH-and T-type bacteria each require type-specific salts for the optimum oxidation of succinic acid. Type-specificity was also observed at 37°C. To investigate further the type-specificity of the salt requirement, the succinic acid and fumaric acid oxidations at 25°C and 37°C were examined, using five strains of M-type. The results showed the universal validity of type-specificity in the salt requirement. Type-specificity was markedly stable, when cells were acclimated in the minimum salt medium for six months.