シロサケ脳下垂体性腺刺激ホルモンの精製

抄録

Purification of chum salmon gonadotropin was attempted. The pituitary glands of chum salmon were extracted with 40% ethanol. Ethanol was added to the extract to give a final 85% solution. The resulting precipitate was collected by centrifugation and dried with acetone. The crude gonadotropin powder obtained was filtered through a Sephadex G-100 column using 0.1_M NH₄HCO₃, pH 8.3, resulting in the appearance of 3 peaks. Gonadotropic activity as assayed by the spermiation test was recognized only in the second fraction (80%response). This gonadotropic fraction (CSG•G100) was lyophilized and chromatographed on a DEAE-cellulose column with a linear gradient. The active fraction (100% response) was eluted in 0.165_M NaCl-0.001_M Tris-HCl, pH 7.0. When eluted stepwise with 0.08, 0.16, 0.24_M NaCl, the gonadotropin (CSG•DEAE) was eluted at 0.16M. The gonadotropin exhibited a single band on polyacrylamide gel disc electrophoresis at pH 9.4.