海藻のクロロフィルに関する研究-I

乾燥ワカメ抽出液の吸収スペクトルとクロロフィル含量

抄録

The dried product of sea-weed Undaria pinnatifida (dried ‘Wakame’ product) which has a deep black color has been judged to be of high quality. In this paper, the relations between the quality and chlorophyll contents in dried ‘Wakame’ products were investigated. The following results were obtained.
1. The absorption maxima of the pigments extracted from good quality specimens with 80% acetone shifted into the range of 660.5-662nm for the “red” maxima and 425-427nm for the “blue” maxima, giving the blue/red absorbance ratio of 1.73-1.94. In inferior quality specimens the absorption maxima were 666-667nm and 406-409.5nm with the blue/red absorbance ratio of 2.65-2.97.
Thus, it was evident that the spectrophotometric properties of pigments extracted from good quality specimens were similar to chlorophyll a, although the absorbance ratio was larger than for chlorophyll a (1.24-1.28); pigments of inferior specimens were similar to pheophytin a, although the absorbance ratio was larger than for pheophytin a (2.03-2.08).
2. Larger amounts of chlorophyll a were contained in good quality specimens (107.9-268.5mg/100g) compared with the amounts in inferior specimens (0-34.2 mg/100g); the contents of pheophytin a (0-70.2mg/100g) in good specimens were smaller than in the inferior specimens (154.4-645.1mg/100g).