凍結乾燥筋原繊維タンパク質の変性に及ぼす相対湿度の影響
1983 年 49 巻 4 号 p. 627-632
詳細
1983 年 49 巻 4 号 p. 627-632
The influence of relative humidity (RH) on the quality change of lyophilized carp myofibrils during storage was estimated. Carp myofibrils were ground with polyphosphates and sucrose. The mixtures were freeze-dried at 60 or 35°C, ground into powder, and stored in desiccators and over saturated salts slurries and other materials with constant relative humidities. Moisture sorption isotherms for lyophilized carp myofibrils were determined by drying those stored for ten days in desiccators. At suitable intervals, aliquots of the powders were taken out and examined as to the quality, using as indexe: solubility in 0.6 M KCl, relative viscosity and ATPase activity of 0.6 M KCl extractable protein, and the total ATPase activity.
The results for powders with RH 10 to 98% stored at room temperature of 15 to 18.8°C for 6 months indicated that the lower the humidity, the better was the quality retention of the powder. In RH 10 to 15% there was no significant difference in the effect of RH. A second experiment studied RH 0 to 20% the qualities of powders stored in the RH below 15% were acceptable for 1 year at 20°C. The last experiment was concerned with RH 0 to 20% at a high temperature of 35°C; here, storing in th RH below 10% gave the qualities which were acceptable for a storage period of 6.5 months. Thus it was shown that the lyophilized myofibrils were stable even in the moisture range of free from monolayer moisture.
