ニジマスにおけるプロリル・ヒドロキシラーゼの分布と二,三の性質
1984 年 50 巻 6 号 p. 1009-1013
詳細
1984 年 50 巻 6 号 p. 1009-1013
Distribution and some properties of prolyl hydroxylase (EC 1.14.11.2) which catalyzes the hydroxylation of proline in a polypeptide precursor of collagen were studied in rainbow trout.
The enzyme activity was found to be present in various tissues of rainbow trout. The specific activity was highest in the centrum. Relativity high specific activity was also found in the swimblakdder, skin, and gill. The enzyme was located the microsomal and soluble fractions of the liver. The enzyme from th liver required α-ketoglutaric acid, ascorbic acid and ferrous ion for activity. The optimum temperatrue for the activity of the enzyme from the rainbow trout liver was around 20“C, whereas that of the enzyme from the carp hepatopancreas was around 25”C. The differece in the optimum temperature for the activity of the enzyme of these two fishes might be related to their optimum living temperatures.
