The effect of NaCl on the production of bacteriolytic enzyme by a bacterium isolated from coastal waters was investigated using polypepton-yeast extract medium.
The production of lytic enzyme was not enhanced by the combination of NaCl and KCl, while Nacl and KCl is respectively effective for the enzyme production. Strain V 37 cells grown in the medium without added NaCl for 3-6 hours produced extracellular lytic enzyme immediately after Nacl was added into the growth medium. However, the stationary-phase cells could not produce lytic enzyme by the addition of Nacl. Strain V 37 cells grown in the medium containing NaCl could not produce extracellular lytic enzyme considerably after transferred into the medium without added NaCl. Strain V 37 seemed to require NaCl for the enzyme production during the active growth.
