自由遊泳時の心電図測定装置の考案

抄録

In order to investigate the autonomic background of behavior in fish, a new method for recording ECG R-spike under unrestrained condition was devised. At first, the positional behavior of bitterling Rhodeus ocellatus ocellatus was studied in a test pool composed of 4 canals arranged in parallel with different water flows, and the condition under which a fish stayed swimming at the same position in a canal was clarified.
Secondly, in a circulation canal consisting of 4 chambers connected in series, electrodes were set at the bottom on the location where the fish preferred to stay swimming. The R-spike recorded was 30-360 μV in amplitude according to the distance between fish and electrode. It was possible to record simultaneously the opercular movements as well as R-spikes.
Furthermore, two canal systems of the same size and shape as the original one were set bilaterally that allowed water passage among them through holes. Thus the fish was able to perceive each other through transparent wall with holes by the sense of sight and smell etc., and the social influences of species mates were possible to be studied.