腹腔内注入による下痢性貝毒のマウステストにおける遊離不飽和脂肪酸の毒性
1984 年 50 巻 8 号 p. 1413-1418
詳細
1984 年 50 巻 8 号 p. 1413-1418
The acetone extracts from three samples of the hepatopancreas of the poisonous scallops obtained on the Okhotsk Coast of Hokkaido Island were fractionated into two parts, hexane soluble fraction (fraction H) and 85% aqueous ethanol soluble fraction (fraction E) by partition to two layers. The majortoxic components in the mouse assay of “diarrheic shellfish toxin” by intra-peritoneal injection were found to be free unsaturated fatty acids showed the following toxicity in MU per g, 18:1 n-9 35, 18:2 n-6 83, 18:3 n-3 167, 18:4 n-3 83, 20:5 n-3 167, and 22:6 n-3 83, respectively. Toxicity of the fraction Hin MUper g was much lower than that of the fraction E. However, the toxicity of the fraction H per 1 g of the hepatopancreas was about twice that of the fraction E, since the fraction Hwas much more abundant than the fraction E in the hepatopancreas. The method for the assay of the diarrhetic shellfish toxin must be reexamined by considering the toxic effect of the free unsaturated fatty acids.
