ヤマメ胚肝由来細胞株の樹立と生物学的性状

抄録

We tried to cultivate fish liver cells using the embryonated eggs of Yamame, landlocked form of masu salmon, Oncorhynchius masou. A continuous cell line designated YEL-13 was established and has been subcultured more than 120 times over the period of 3 years. YEL-13 cells grow in the manner of contact inhibited monolayer and consisted of epithelial cells and a few fibrobrastic cells. Electron microscopically, most of the cells had a large and round nucleus and well-develop-ed rough surfaced endoplasmic reticulum in cytoplasm and smooth cell surface with poorly de-veloped microvilli. Chromosme number of YEL-13 ranged widely and most of them were be-tween 50 and 83. The cells with 56 chromosomes occupied 24% of those examined. YEL-13 cells have the permissible temperature range of 15-20°C for growth. The cell line has been producing and relaesing a Yamame serum protein constantly which is one of the main functions of liver parenchymal cells.