魚類の抗体および補体の種間適合性

抄録

In order to examine species compatibilities of antibody and complement, hemolytic reactions were carried out adopting sheep erythrocytes sensitized with carp, tilapia, yellowtail, porgy or rabbit antibody in combination with sera of rainbow trout, ayu, carp, tilapia, porgy, yellowtail or guinea pig. None of the fish sera were potentiated by rabbit lgM, and the guinea pig comptement did not react with fish antibodies. Some compatibilities were observed between antiboby and complement of different fish species; rainbow trout, ayu and porgy complement were potentiated by porgy, carp and tilapia antibody, respectively.
The hemolytic activity of fish sera was lost by carrageenan-, ammonia-and heat-treatment, and the combination of ammonia-treated serum with carrageenan-treated serum (Cn) or heat-treated serum resulted in restoration of the hemolytic activity. This indicates that these fish sera contain the complement components C1, C4 and C2.
Hemolytic intermediate complex, EACl, was incubated with Cn (serum deficient in C1) from different species to examine the species compatibility between C1 and C4. As a result, no compatibility was observed between the guinea pig and fish. Furthermore, in the majority of the combinations of C1 and Cn of different fishes, hemolysis were not induced; interspecies compatibilities were seen in only two combinations (porgy C1+tilapia Cn and tilapia C1+porgy Cn).