ポルフィラン分解酵素産生細菌の分離と同定
1990 年 56 巻 5 号 p. 819-823
詳細
1990 年 56 巻 5 号 p. 819-823
A screening was undertaken to acquire bacteria with porphyran-degrading enzyme productivi-ty. Several strains were obtained from the sea environment in Fukuoka Prefecture. Among them, the strain AP-2 from an alga showed the highest productivity of the enzyme. The organism needed the extract from P. yezoensis as an inducer to produce porphyran-degrading enzyme, and released a large amount of the enzyme into the culture fluid, when incubated with shaking at 25°C for 5 days in the following liquid medium; 0.5% peptone, 0.1% yeast extract, 3% NaCl in the extract from P. yezoensis, pH 7.4. The enzyme exhibited a high activity at pH 6.5. The enzyme seems to be an β-agarase which cleaves not only porphyran but also agar to give various sizes of neoagaro-oligosaccharides.
According to criteria of the Bergey's Manual of Systematic Bacteriology, Vol. 1, the bacterium was assigned to the genus Vibrio, but not listed species name for this organism.
