カフェインと高温処理によるアコヤガイ三倍体の誘導

抄録

A combination of caffeine treatment with heat shock treatment on eggs was examined to induce triploidy in the pearl oyster Pinctada fucta martensii. At 31.5°C, different concentrations from 1m_M to 13m_M of caffeine were applied to eggs before the second meiosis. The increase of caffeine concentration induced an increasing delay or abnormality of metamorphosis from embryo to D-shaped larvae. Histograms of DNA-content in nuclei of cells from D-shaped larvae showed that the highest percentage (35.8%) of triploid D-shaped larvae was induced by 13m_M caffeine.