Three scale (A, B, C) samples from a red sea bream were analyzed two dimensionally with a scan covering approximately 3000 spots, for a time duration of 15000s using 16.5keV monochromatized synchrotron X-rays focussed into an area of 150×200μm2 by a double crystal monochrometer.
General features of elemental distribution were similar between scales A and C. Sr concentration was characteristic on the scales, and its pattern was similar with that of Ca, with a higher concentration at the focus and along grooves. Zn did not show any specific elemental locality over the scales.
Scale B showed elemental locality significances. The elemental distribution of Zn around the lateral line pore of the scale was characteristic, as was the distribution of Ca and Sr. The ratio of Sr to Ca especially along the grooves and focus was observed to be significantly high, about 4.7, which is approximately 20 times higher than the value generally accepted.
