高水温処理によるギンブナ卵へのコイ精子の導入とDNAマーカーによる確認
1997 年 63 巻 2 号 p. 201-206
詳細
1997 年 63 巻 2 号 p. 201-206
The eggs of a Ginbuna Carassius langsdorfii (all female triploids) were inseminated by the sperms of a common carp Cyprinus carpio. A part of the eggs was treated by heat shock: 40°C for 1.0min at 5min after fertilization.
The average of erythrocytic major diameter of offsprings from non-treated eggs was 17.4μm (17.1-17.7μm). The patterns of glucosephosphate isomerase (GPI), RAPD-PCR (random primer: OPA-04, 09, 15), and DNA fingerprinting (restriction endonuclease: Hae III; probe: YNZ 22) in the offsprings were identical with maternal fish.
The average of erythrocytic major diameter of offsprings from treated eggs was 20.6μm (20.3-20.9μm). The patterns of GPI, RAPD-PCR, and DNA fingerprinting in the offsprings were composed of all bands of maternal fish and partial bands of paternal fish.
Therefore, the results obtained in this study suggest that the offsprings from non-treated eggs were triploid clones, and the offsprings from treated eggs might be tetraploid composed of genome from both parents.
