Abstract
(1) The separation procedure of adenosine from plasma using paper chromatography described by Berne (Amer. J. Physiol. 1963, 204, 317-322) is analysed for the recovery rate and for the sources of loss. When adenosine-8-14C is added to plasma, the recovery rate of total 14C from the paper chromatogram is 87.7±5.4% (mean±S. D.). This rate is not influenced by the concentration of non-radioactive adenosine. About 40% of adenosine is broken down during the procedure, even when the plasma is precooled at 0°C. (2) The recovery rate of radioactivity is 75.6±3.2% in blood cells deproteinized with perchloric acid. (3) The incubation study of the radioactive adenosine with whole arterial blood reveals that the recovered 14C is 9.29±1.41% in the adenosine spot derived from the plasma. (4) Theoretical estimation of the total amount of adenosine released from an organ is offered with the consideration of the amounts broken down in plasma and uptaken into blood cells, and of the amount re-uptaken into the organ.
