The Tohoku Journal of Experimental Medicine
Online ISSN : 1349-3329
Print ISSN : 0040-8727
ISSN-L : 0040-8727
Specific Role of Magnesium Ion in Protecting the Anticomplementary Effect of DNA in Immune Hemolysis
EIJI OKUHARA, JUNKO IGARASHI, AKIRA WAKIZAKA, TSUTOMU MATSUMOTO
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Keywords: DNA, complement fixation
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1975 Volume 116 Issue 1 Pages 33-37

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Abstract

When a constant quantity of denatured DNA required for C'-fixation reaction was employed as the antigen, an anticomplementary action of DNA was frequently observed. In order to cope with this difficulty in the analysis of C'-fixation, the relationship between DNA, Mg2+ and C' was investigated. It was found that this phenomenon was closely related to the flexible conformation of DNA which is highly influenced by Mg2+. Further, this effect could be eliminated in the presence of 5mM MgCl2. The mechanism of the elimination by Mg2+ of the anticomplementary effect of DNA was discussed.

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