Fourteen pancreatic calculi from the corresponding number of pancreatic lithiasis patients were examined mineralogically and histochemically. The following results were obtained. The main components of calculi were calcium carbonate in 13 of the 14 cases, and calcium phosphate in the remaining one. Calcium carbonate calculi were all so-called intraductal calculi, with acidic gly-coprotein apparently enwrapping the component particles. Acidic glycoproteins acted to bridge calcium carbonate particles, as in the cases of gallstone and urinary stone. The calcium phosphate calculus had a histochemical feature of pathologic calcification with degenerated collagen fibrils as the matrix, suggesting the calcification of the fibrotic pancreatic parenchyma after pancreatitis.
Since the half-life of 99mTc is only 6.04hr, its massive dosing for an elevation of diagnostic accuracy is possible with reduced radiation exposure to the body. On 11 impotent cases, radioisotope penogram was performed with 99mTcO-4. The results indicate that the penogram curves obtained are satisfactorily favorable for diagnostic discrimination between organic impotence and functional impotence.
The physical growth, the development of orienting behavior and neuromotor reflexes of offspring of rats that received an irradiation of a diagnostic level of ultrasound on the 9th day of gestation were estimated and compared with those of two control groups (untreated control and sham-irradiated or immobilized control). Results showed no significant group differences in terms of either physical development or development of orienting behavior. In the reflexological tests, however, a number of those reflexes that developed after 6 days of postnatal life of the offspring of irradiated rats showed significant delays in maturation when compared with those of the untreated control rats, but showed no difference from those of the sham-irradiated group. From these findings it is suggested that under certain circumstances such as in stress, the prenatally irradiated ultrasound of even a low-intensity may affect the development of the brain of offspring.
Serum alkaline phosphatase activity was found to increase more markedly in patients with liver cirrhosis than in patients with peptic ulcer and this increase was found to be influenced by blood types. After testing several amino acids and bile acids, phenylalanine and cholic acid were chosen and their inhibitory effects upon serum alkaline phosphatase activity were studied in 66 patients with various liver diseases. It was found that the combination of both agents demonstrates different patterns of inhibition between the patients with liver cirrhosis and obstructive jaundice. This inhibitory effects were also variable among cases of different blood types. Basing upon the present observation, the possible source of the elevated alkaline phosphatase activity in liver cirrhosis was discussed.
When a constant quantity of denatured DNA required for C'-fixation reaction was employed as the antigen, an anticomplementary action of DNA was frequently observed. In order to cope with this difficulty in the analysis of C'-fixation, the relationship between DNA, Mg2+ and C' was investigated. It was found that this phenomenon was closely related to the flexible conformation of DNA which is highly influenced by Mg2+. Further, this effect could be eliminated in the presence of 5mM MgCl2. The mechanism of the elimination by Mg2+ of the anticomplementary effect of DNA was discussed.
The kinetics of catalase synthesis and degradation were determined in acatalasemia and normal mouse livers during recovery of catalase activity after inhibition with aminotriazole. Catalase turned over with a half life of 10.0hr in acatalasemia mice and of 19.2hr in normal mice. The rate of catalase synthesis (KS) was 0.168 (PU/mg protein/hr) in acatalasemia mice and 0.271 in normal mice. The rate of catalase degradation (KD) was 0.069 and 0.036 per hour in acatalasemia and normal mice, respectively.
The cow snout epidermis consists of two different parts. The one has the granular cell layers, and the other not. Electron-microscopically, the generation of the lamellated granules and the thickened cellular envelope of a horny cell were noted in both regions. In the horny cell layer the remnant of the lamellated granules were observed locating in the intercellular space, that is, outside of the thickened cellular envelope. The thickened cellular envelope was generated by the precipitation of electron dense material along the inner leaflet of the plasma membrane, but neither by the attachment of small keratohyalin granules to the plasma membrane nor by the coating of the plasma membrane with the content of lamellated granules.
In a hypouricemic and mentally retarded infant due to a defect of 5-phosphoribosylpyrophosphate synthetase, electroencephalograms were recorded at the age of 4, 7, 10 and 11 months. Hypsarrhythmia was first observed at the age of 10 months, and markedly improved after ACTH therapy with concomitant increase in the enzyme activity of erythrocytes.
It was attempted to describe the morphology of the most immature cell of megakaryocytic series. The megakaryocytes were observed with the electron microscope in five cases, being traced back to their immature forms. In two cases the most immature cells of megakaryocytic series were considered to be the cells which were probably identified as lymphocytes under the light microscope, but they were not lymphocytes with the electron microscope. In other two cases it was presumed that neutrophilic and megakaryocytic series were derived from morphologically similar immature cells, since the most immature cells of neutrophilic and megakaryocytic series were not distinguished when they were traced back to their immature forms. These findings suggest that mother cells of mega-karyocytes in the adult bone marrow may be identified as lymphoid cells with the light microscope.
Megakaryocytes from 5 patients and 1 normal person were observed electronmicroscopically. In some pathologic states platelets seemed to be liberated without demarcation membrane system (DMS) and in a normal individual they seemed to be liberated independently of DMS. These findings suggest that DMS is not concerned with platelet liberation and that platelets are liberated through pseudopodia and bleb formation. In mature megakaryocytes vigorous amoeboid movement seems to exist and both pseudopodia and blebs may represent this movement. Structural similarity between surface connected system (SCS) of platelet and DMS of megakaryocyte suggests that the structure called DMS is transported as SCS into platelet.
Immunosuppressive effect of pregnant serum obtained in the second and third trimesters was demonstrated in the PHA-induced homologous lymphocyte stimulation system, where 3H-thymidine in-corporation into DNA was measured. Such an inhibitory factor was not present in the first trimester serum. The immunosuppressive principle detected in the second trimester serum was characterized by a potent inhibitory activity even at an increased concentration of PHA up to 75μg, whereas the inhibitory activity found in the third trimester serum at optimum PHA concentration (15μg) was not detectable when the PHA concentration was raised to 75μg. Thus, the second and third trimester sera must contain different immuno-suppressive principles.
A new simple test for the screening of pre-, mid- and post-menopausal women with unidentified complaints overlaid by psychiatric disorders has been devised. This test is performed by intravenous injection of conjugated estrogen and by comparing Kupperman's menopausal indices before and after the injection. That is, the discriminant function between groups with and without psychiatric abnormalities was obtained from Kupperman's menopausal indices before and after intravenous injection of conjugated estrogen, and from this function was made a calculative differential diagnosis between the two groups. The calculative diagnosis agreed fairly well with the clinical diagnosis by the psychiatrist.
Hydroxyproline content was chemically determined on 45 human hearts obtained at autopsy in order to study whether hydroxyproline contents vary in proportion to heart weight or not. There were no significant differences in the mean hydroxyproline content between the epicardium and the endocardium, between the left ventricle and the right ventricle, or among cardiovascular and renal disease group, malignant disease group and miscellaneous disease group. Total hydroxyproline content per mg dry weight of the left ventricle increased proportionally with heart weight. Hydroxyproline content in neutral salt soluble fraction increased more proportionally than that in acid soluble fraction. This tendency was more evident in the endocardium than in the epicardium of the left ventricle. These results seem to be well consistent with those of histometrical estimation of scar tissue in human heart muscle. Hydroxyproline content in acid soluble fraction of the right ventricle was increased proportionally with heart weight.
Although many cases of hepatitis associated with aplastic anemia have been described, the nature of hepatitis is not fully understood. There has been so far no report of hepatitis-B-antigen-positive case. A case of acute viral hepatitis B developing aplastic anemia is presented.