Abstract
The factor VIII response to DDAVP (1-deamino-8-D-arginine vasopressin) was examined in 6 healthy volunteers. Following intravenous DDAVP infusion, each subject showed a marked increase in factor VIII procoagulant activity (VIII: C), factor VIII related antigen (VIIIR: AG) and von Willebrand factor activity (VIIIR: WF). But there were differences among augmentation index of each activity. Further investigations were performed whether there was a qualitative abnormality in the factor VIII after DDAVP infusion. Between factor VIII activities before and after DDAVP, there was no difference in a thermostability of VIII: C in vitro, gel chromatographic pattern on Sepharose 2B or 4B, a precipitation with con-canavalin A (Con A) and a recovery in cryoprecipitate. The increase of VIIIR: AG, however, was mostly brought about by the augmentation of relatively slowly migrating portion as demonstrated by crossed immunoelectrophoresis (CIE). From these results it was assumed that there was no remarkable abnormality in the factor VIII after DDAVP administration, and the elevated factor VIII could function efficiently in vivo. The findings in cryoprecipitation showed that DDAVP could be applied to blood donation for the preparation of cryoprecipitate.
