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Volume 132 (1980)
- Issue 4 Pages 369-
- Issue 3 Pages 245-
- Issue 2 Pages 123-
- Issue 1 Pages 1-
Volume 132, Issue 2
Displaying 1-18 of 18 articles from this issue
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KEIYA TADA, KUNIAKI NARISAWA, NOBUHIRO ARAI, YOSHIHIKO OGASAWARA, SHIN ...1980 Volume 132 Issue 2 Pages 123-131
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThree siblings with hyperphenylalaninemia were described, who showed convulsions and severe mental retardation despite dietary control of blood phenylalanine. The cultured skin fibroblasts from two patients revealed a markedly low activity of dihydropteridine reductase. These patients showed low levels of serum folate and of 5-hydroxyindoleacetic acid and homovanillic acid in cerebrospinal fluid. The postmortem examination on one of these siblings exhibited the dysmyelination in the cerebrum and spongy lesions of the white matter in the cerebellum, pons, medulla oblongata and spinal cord. Many minute foci of calcification were found in the cerebrum, which were located in the perivascular space of the small blood vessels. Such calcification was observed on CT scanning of the brain in the other two living siblings as well.View full abstractDownload PDF (2797K) -
HOYU TAKAHASHI, MASAKAZU ITOH, ISAO KOBAYASHI, NOBUO SAKURAGAWA, AKIRA ...1980 Volume 132 Issue 2 Pages 133-140
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe factor VIII response to DDAVP (1-deamino-8-D-arginine vasopressin) was examined in 6 healthy volunteers. Following intravenous DDAVP infusion, each subject showed a marked increase in factor VIII procoagulant activity (VIII: C), factor VIII related antigen (VIIIR: AG) and von Willebrand factor activity (VIIIR: WF). But there were differences among augmentation index of each activity. Further investigations were performed whether there was a qualitative abnormality in the factor VIII after DDAVP infusion. Between factor VIII activities before and after DDAVP, there was no difference in a thermostability of VIII: C in vitro, gel chromatographic pattern on Sepharose 2B or 4B, a precipitation with con-canavalin A (Con A) and a recovery in cryoprecipitate. The increase of VIIIR: AG, however, was mostly brought about by the augmentation of relatively slowly migrating portion as demonstrated by crossed immunoelectrophoresis (CIE). From these results it was assumed that there was no remarkable abnormality in the factor VIII after DDAVP administration, and the elevated factor VIII could function efficiently in vivo. The findings in cryoprecipitation showed that DDAVP could be applied to blood donation for the preparation of cryoprecipitate.View full abstractDownload PDF (961K) -
HOYU TAKAHASHI, NOBUO SAKURAGAWA, AKIRA SHIBATA1980 Volume 132 Issue 2 Pages 141-146
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSPrecipitation pattern of factor VIII related antigen (VIIIR: AG) with concanavalin A (Con A), a glycoprotein binding lectin, was investigated in normal subjects and patients with a variant of von Willebrand's disease (VWD) and disseminated intravascular coagulation (DIC). Decreased precipitation with Con A was demonstrated in patients with a variant of VWD in which VIIIR: AG showed an increase in electrophoretic mobility on crossed immunoelectrophoresis (CIE). Similar findings were obtained in patients with DIC which revealed normal electrophoretic mobility of VIIIR: AG. This procedure was assumed to be useful for the detection of a qualitative abnormality of the factor VIII protein.View full abstractDownload PDF (936K) -
MASAHIKO ENDO, ZENSAKU YOSIZAWA1980 Volume 132 Issue 2 Pages 147-152
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe endometrial scrapings obtained from the uteri of estrogen-progesterone-treated and estrogen-treated rabbits were incubated with N-acetyl-D-[1-3H] glucosamine and [35S] sulfate, and then the incubation medium (M-Fr) was separated from the tissue. The tissue was subsequently homogenized exhaustively in 0.25M sucrose, and the insoluble residue (R-Fr) was separated. The supernatant at 8, 5000×g for 10min of the homogenate was subjected to subcellular fractionation by discontinuous sucrose gradient ultracentrifugation, and a Golgi-rich fraction (G-Fr) was obtained. Crude glycoconjugates (glycosaminoglycans and glycoproteins) were then separated from M-Fr, R-Fr and G-Fr after pronase digestion. The amounts of the radioactivities incorporated into these glycoconjugates suggested that progesterone markedly suppressed the estrogen effect within 6hr in R-Fr and G-Fr, whereas the suppression slightly delayed in M-Fr. The amounts of the radioactivities incorporated into acidic GC, which obtained by CPC-precipitation of the crude GC after digestion with crude heparinase, indicated that the biosynthesis of the CPC-precipitable sulfated glycoproteins was almost completely ceased by progesterone, although other glycoconjugates were still actively synthesized under the progesteronic condition.View full abstractDownload PDF (361K) -
RYUICHI NAKAMURA, REIJI TANIGUCHI1980 Volume 132 Issue 2 Pages 153-158
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSPremotor times (PMTs) of both biceps muscles in flexion and supination of the forearms were examined in 13 patients with Parkinson's disease (PD), 10 with cerebellar degeneration (CD) and 14 control subjects. Compared with the control, PMT of CD increased definitely but that of PD was within normal range. The difference of PMTs between flexion and supination was lost in PD, but not in CD. It is assumed that the basal ganglia and the right cerebral cortex are functioning for the selection of movement patterns at the initiation of movements.View full abstractDownload PDF (319K) -
YASUKO NAGATSUKA, KATSUMI SATO, NOBORU OTOTANI, ZENSAKU YOSIZAWA1980 Volume 132 Issue 2 Pages 159-171
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSTo obtain a clue for the metabolic disorder of glycosaminoglycans (GAG) in connective tissue diseases (CTD), a device of screening test for excretion pattern of urinary GAG was achieved. The crude GAG were separated from voluntary 100-20ml urine by precipitation with cetylpyridinium chloride, followed by digestion with pronase P. The crude GAG were then separated by one-dimensional Separax (cellulose acetate membrane) electrophoresis in 0.2M aqueous calcium acetate. GAG, in terms of hexosamine, in each GAG-band were quantitated after extraction from the strip with water. All the urines examined gave three regular bands, bands 1, 2 and 3, but urines from most of the pupils at puberty and from several adults gave irregular bands, besides the regular ones. As for the regular bands, a significant difference was observed in percent distribution of bands 1, 2 and 3 between the samples from male and female of the normal youth aged 20-29, and excretion pattern of three regular bands reached to an adult type much younger in female than in male. GAG in the regular bands were identified as follows: band 1, partially desulfated chondroitin sulfates A and C; band 2, dermatan sulfate plus low-sulfated chondroitin sulfates (LSC) in most cases; band 3, heparan sulfate plus LSC in most cases. Usefulness in clinical field of the screening test is indicated.View full abstractDownload PDF (714K) -
TATSUHITO TONO-OKA, MASAYUKI NAKAYAMA, MASAHITO OHKAWA, SHUZO MATSUMOT ...1980 Volume 132 Issue 2 Pages 173-178
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSChemotactic responses of patients with a variety of diseases to two different types of chemotactic factors were analyzed. Granulocytes were obtained from the patients with childhood malignant diseases during chemotherapy, the patients with measles, the adult patients with recurrent aphthous stomatitis, and newborn infants. 22 out of all the 38 patients (58%) showed decreased chemotactic response to zymosan activated human serum (ZAS), whereas only 9 patients (24%) showed decreased response to Escherichia coli-derived chemotactic factor (bacterial chemotactic factor: BCF). Furthermore, only 6 out of 22 patients who showed decreased chemotaxis to ZAS showed decreased chemotaxis to BCF. Analogous results were also obtained with cord blood granulocytes. These facts suggest that various kinds of chemotactic factors activate granulocytes in different ways, and that the evaluation of chemotactic response in a patient should be made simultaneously using more than two different types of chemotactic factor.View full abstractDownload PDF (369K) -
HIROSHI KANEDA, TOSHIAKI YAMAUCHI, TOYOAKI MURATA, JUN MATSUMOTO, TAKE ...1980 Volume 132 Issue 2 Pages 179-186
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSA man, now 46 years of age, 159cm in height and 39kg in weight, was diagnosed five years ago as essential hypertension with hypertensive nephropathy. On that occasion, renovascular stenosis was ruled out angiographically. Recently, he developed malignant hypertension and was admitted to our hosptial. On admission, severe hypertension (260/160mmHg), hyponatremia (132mEq/liter), high reninemia (40.0ng/ml; normal, 5.0-30.0ng/ml), high serum aldosterone level (840pg/ml; normal, 10-100pg/ml) and advanced retinal changes (KWIII) were found. He lost 10kg in weight during the preceding 3 months. Other characteristic laboratory findings were hypopotassemia (3.8mEq/liter), hypochloremia (85mEq/liter), slight alkalosis, high serum lactate dehydrogenase level, increased cardio-thoracic ratio, and mild azotmia (BUN-46mg/100ml, serum creatinine-3.8mg/100ml). As a therapeutic trial, β-blocker and hydralazine were administered, but no improvement was obtained. Then, sodium was infused in a dose of 328mEq dissolved in 1, 500ml water in a time period of 12hr. One hr after the start of sodium supplementation, his blood pressure was dramatically decreased to 174/120mmHg; 3hr after the infusion, the blood pressure was 140/90mmHg; one hr after the end of the infusion, the blood pressure remained as low as 130/90mmHg. At the end of the infusion, serum sodium rose to 141mEq/liter, PRA decreased to 8.0ng/ml, serum aldosterone level fell to 250pg/ ml, urinary sodium excretion decreased, and body weight increased by 1.5kg. From these findings, it seems likely that in this patient extreme hypertension caused an excessive diuresis resulting in fluid and sodium depletion in the body; the latter, in turn, eliciting further increase in renin release and blood pressure elevation. It may be concluded that malignant hypertension with hyponatremia and emaciation is an indication of parenteral sodium supplementation, and that the most important in the control of malignant hypertension is to keep the sodium balance in an appropriate range.View full abstractDownload PDF (434K) -
TOYOHARU ISAWA, TAKEO TESHIMA, TOMIO HIRANO, KOICHIRO SHIRAISHI, TAKAS ...1980 Volume 132 Issue 2 Pages 187-197
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe effect of alveolar hypercapnia under a wide range of alveolar oxygen tensions was assessed in the right upper lobe of the normal dogs and the dogs with the reimplanted right lung. To induce alveolar hypercapnia by artificial ventilation of the right upper lobe, gas mixtures of differing oxygen fractions containing 10% or 20% CO2, were used as exchange gases for the right upper lobe. The concurrent alveolar hypercapnia had little superimposed effect on the regional vascular responses to a wide range of alveolar oxygen tensions in the normal and the reimplanted denervated lung lobe. Under an extreme alveolar hyperoxia, however, the concurrent alveolar hypercapnia somewhat suppressed regional hyperoxic vasodilation and/or recruitment of the pulmonary vascular beds, whereas in the reimplanted lung lobe it potentiated the hyperoxic pulmonary vascular response. The alveolar oxygen tension seems to be the main factor in the regulation of regional perfusion in the lungs.View full abstractDownload PDF (672K) -
NOBORU MATSUMOTO, YUKIO HIROSHIGE1980 Volume 132 Issue 2 Pages 199-205
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSShape changes of GSH-treated human erythrocytes during the acidified serum test were examined by a differential interference microscope. A time-course study of the erythrocytes undergoing complement-mediated hemolysis revealed two kinds of hemolytic processes. One is rapid hemolysis, in which GSH-treated erythrocytes are transformed into spherical forms prior to hemolysis. The other process is gradual or slow hemolysis of a small proportion of GSH-treated erythrocytes. In this process, the erythrocytes are transformed into echinocytes and then into spheroechinocytes prior to hemolysis. However, echinocytic shape changes are not an absolute prerequisite to hemolysis, since echinocytes are not always transformed into a prelytic spherical form, but some of them are reversed to discocytes. Under the experimental conditions of this study, rapid hemolysis of spherical erythrocytes is the main mechanism of hemolysis.View full abstractDownload PDF (2435K) -
TOKUTARO SATO, SHIRO OGUMA, TAKAO SAITO, KAORU YOSHINAGA1980 Volume 132 Issue 2 Pages 207-212
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSA method was established to isolate the glomeruli from the kidney fixed in formaldehyde. O-Hydroxylysylglycosides and hydroxyproline in the isolated glomeruli from the kidney of normal subjects, diabetics and subjects with chronic glomerulonephritis were measured on the alkaline hydrolyzate of the glomeruli. O-Hydroxylysylglycosides in 1000 glomeruli were 9.42±2.68 nmoles (mean±s. D. ) for controls, 12.92±7.36 nmoles for diabetics and 12.54±4.62 nmoles for subjects with chronic glomerulonephritis. Hydroxyproline in 1000 glomeruli from control, diabetics and subjects with chronic glomerulonephritis was 43.0±10.7, 82.0±39.3 and 52.1±21.3 nmoles, respectively. Hydroxyproline in the hyalinized glomeruli from diabetics was 123.3±22.4 nmoles/1000 glomeruli, and significantly increased in comparison with that in the glomeruli from chronic glomerulonephritis or control subjects.View full abstractDownload PDF (856K) -
AKIRA SATO, KUNIHARU ISHIOKA, MITSUO KOBIYAMA, MASASHI GOTO, SHIGERU A ...1980 Volume 132 Issue 2 Pages 213-223
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe cytological findings in 7 cases of leiomyoma and 4 cases of leiomyosarcoma can be summarized as follows: For differential diagnosis of these lesions, in comparison with leiomyoma cells, leiomyosarcoma cells were found to have 1) increased minor axis diameters of the nuclei and nuclear anisokaryosis, 2) dark nuclear staining, 3) enlarged and darkly stained chromocenters, 4) dark staining and thickening of the nuclear rim, 5) enlargement of nuclear clear areas, 6) increased numbers of oval nuclei and greater pleomorphism, 7) an increase in size and number of the nucleoli, 8) a strong tendency for cell atypism, such as anisocytosis and pleomorphism. In the light of these findings, it is believed that differential diagnosis is indeed possible.View full abstractDownload PDF (2516K) -
AKIRA YAJIMA, HISASHI HIGASHIIWAI, AKIRA SATO, MASAAKI WATANABE, TOSHI ...1980 Volume 132 Issue 2 Pages 225-229
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSAmong the 95 cases of ovarian cancer treated by us between May, 1975 and August, 1978, only 33 were suitable for complete resection. The remaining 62 cases underwent incomplete resection, followed by F. Q. C. combination chemotherapy (1-(2tetrahydrofuryl)-5-fluorouracil, carbazilquinone, cytosine arabinoside); 54.8% showed an antitumor response. The median survival time of the responders was 13.2 months, whereas it was 7.4 months for the non-responders. The survival rate after 24 months, however, was 13% and 4%, respectively. Side effects of the drugs, including leucopenia, thrombocytopenia, nausea and vomiting, were found in roughly one half of the cases.View full abstractDownload PDF (305K) -
RIKIYA ABE, AKIO HIROSAKI, MICHIO KIMURA1980 Volume 132 Issue 2 Pages 231-236
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSThe present studies were carried out to clarify the relationship between thyroid function and breast diseases. Neither the mean value of radioiodine uptake rate nor thyroxin (T4) level was significantly different in the patients with benign breast diseases and those with breast cancer or in localized and metastasized cancer groups. As these results were obtained by conventional studies, it became necessary to investigate the dynamic changes of pituitary and thyroid hormones after thyrotrophin releasing hormone (TRH) stimulation. There were no significant differences in serum thyroid stimulating hormone (TSH), prolactin, tri-iodothyronine (T3) or T4 level before and after TRH stimulation among the benign disease, localized and metastasized cancer groups.View full abstractDownload PDF (395K) -
ISAMU USUBUCHI, TATSUSUKE SATO, HAJIME KUDO1980 Volume 132 Issue 2 Pages 237-238
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSIn the peritoneal cavity of the rat, Hirosaki sarcoma, an ascites tumor of the rat, acquired a resistance to the essentially effective dose of 100μg/kg of mitomycin C by means of the daily treatment with the ineffective dose of 10 or 1μg/kg of mitomycin C. It is considered that most of Hirosaki sarcoma cells may have gradually acquired a hereditary resistance to mitomycin C in the course of the contact with the drug.View full abstractDownload PDF (683K) -
KAORU SAGISAKA, HIROKO YAMASHITA, MINED IWASA1980 Volume 132 Issue 2 Pages 239-240
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSNon-hemoglobin proteins (NHP) were effectively isolated from red cell lysate by CM-Sephadex chromatography using 0.01 M phosphate buffer pH 6.3. NHP from 180 subjects was analyzed with polyacrylamide gel disc electrophoresis. The individual difference was observed at the intermediately migrating zone after protein staining. Group I had two of the three bands migrating fastly, group II all the three bands, and group III two of the three bands migrating slowly. Incidences of groups I, II and III were 67, 20 and 13 percent, respectively.View full abstractDownload PDF (686K) -
SHOZO NAKAMURA1980 Volume 132 Issue 2 Pages 241-242
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSAmong non-obese students, those consuming daily 44g or more of alcohol showed significantly higher incidences of abnormality in glutamic oxalacetic transaminase (GOT) and γ-glutamyl transpeptidase (γ-GTP). When daily alcoholic intake of 44g or more was combined with obesity, highly significant increase in incidences of abnormality in GOT, glutamic pyruvic transaminase (GPT), and γ-GTP was observed. In normal weight students, even lower range of alcoholic consumption was associated with significant increase in high density lipoprotein (HDL) cholesterol. As compared with normal weight groups, obesity groups showed significantly lower HDL cholesterol, and leanness group significantly higher HDL cholesterol.View full abstractDownload PDF (136K) -
HIROKI WATANABE, KOUSUKE WATANABE, KAZUO SHIINO, SENZO OINUMA1980 Volume 132 Issue 2 Pages 243-244
Published: 1980
Released on J-STAGE: November 28, 2008
JOURNAL FREE ACCESSWe developed a prototype apparatus for microexplosion lithotripsy in the urinary bladder according to our original idea. A model calculus in a pig bladder was successfully crushed by the apparatus and was removed by aspiration.View full abstractDownload PDF (811K)
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