抄録
TSUCHIYA, S., SATO, T., NAKAE, S., KATAMINE, S., KONNO, T. and TADA, K. Epstein-Barr Virus-Induced Precursor B Cell Lines from Patients with Congenital Agammaglobulinemia. Tohoku J. exp. Med., 1983, 140 (2), 133-144 - We have established lymphoid cell lines with diversity of immunoglobulin expression by infection of bone marrow cells of four patients with congenital agammaglobulinemia with Epstein-Barr virus. Immunofluorescent study revealed that the cells of many of these lymphoid cell lines had characteristics of possible precursor B cells, permitting classification as follows: 1) cell lines without any surface or cytoplasmic immunoglobulins (17/28 lines), 2) cell lines which had only cytoplasmic μ heavy chains (line K4 and O2), 3) cell lines with both μ and δ heavy chains in the cytoplasm (line O6 and M5), 4) cell lines which bore surface μ chains and possessed cytoplasmic μ chains but lacked light chain expression (line S5), 5) cell lines which had surface and cytoplasmic δ and λ chains, and secreted the immunoglobulins (line K5), and 6) cell lines which had surface IgM and cytoplasmic μ and light chains, and secreted the immunoglobulins (5/28 lines). Biosynthetic studies with 14C-leucine confirmed the patterns of immunoglobulin expression found by immunofluorescent analysis. These cell lines may represent some stages of B cell development and provide useful information on the pathophysiology of congenital agammaglobulinemia.
