Extraadrenal Immunohistochemical Distribution of Steroid 21-Hydroxylase in Human

Abstract

SASANO, H. and SASANO, N. Extraadrenal Immunohistochemical Distribution of Steroid 21-Hydroxylase in Human. Tohoku J. exp. Med., 1988, 154 (1), 21-28 - Using an antibody against cytochrome P-450 specific for steroid 21- hydroxylase (P-450_C21), extraadrenal distribution and localization of the enzyme were immunohistochemically examined on various normal human tissues. Immunoreactive P-450_C21 was demonstrated in the renal tubular system, especially in distal tubules and cortical and medullary collecting tubules; excretory ducts including intercalated ducts of the pancreas and salivary glands; secretory ducts of the mammary glands. The immunoreactivity for P-450_C21 was not present in the colon and liver. Transepithelial transport of sodium, potassium and hydrogen ions occurs in those sites where P-450_C21 is present and mineralocorticoid receptors are presumed to be present. Relationship of extraadrenal 21-hydroxylase, which produces deoxycorticosterone locally, to a mineralocorticoid action is proposed.