Effect of Porcine Follicular Fluid on LH and FSH Secretion in Rats

REIKO DEMURA; TOMOHARU SUZUKI; SAEKO NAKAMURA; HIROMI KOMATSU; HIROSHI DEMURA

doi:10.1620/tjem.156.157

REIKO DEMURA, TOMOHARU SUZUKI, SAEKO NAKAMURA, HIROMI KOMATSU, HIROSHI DEMURA

Author information

Keywords: inhibin, porcine follicular fluid, FSH, LH, LH-RH

JOURNAL FREE ACCESS

1988 Volume 156 Issue 2 Pages 157-163

DOI https://doi.org/10.1620/tjem.156.157

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Published: 1988 Received: July 06, 1988 Available on J-STAGE: August 31, 2006 Accepted: September 06, 1988 Advance online publication: - Revised: -
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Date of correction: August 31, 2006 Reason for correction: - Correction: ABSTRACT Details: Wrong : DEMURA, R., SUZUKI, T., NAKAMURA, S., KOMATSU, H. and DEMURA, H. Effect of Porcine Follicular Fluid on LH and FSH Secretion in Rats. Tohoku J. exp. Med., 1988, 156 (2), 157-163 - To investigate a mode and a site of action of inhibin on FSH and LH secretion, in vivo and in vitro effects of porcine follicular fluid (PFF) on FSH and LH secretion were studied in male rats. PFF administerd i.v. suppressed basal FSH levels for 1-8hr, but did not alter LH levels. On the contrary, PFF given either 1 or 6hr in advance, suppressed LH-RH stimulated LH responses as well as LH-RH induced FSH responses. In cultured pituitary cells PFF exerted a partial suppressive effect on LH release. PFF suppressed LH release only under the maximal stimulation with 10^-10M LH-RH and 0.1mM 3-isobutyl-l-metylxanthine (IBMX) to a lesser degree compared with uniform and greater suppression on FSH release regardless of the experimental conditions. Above results showed a selective suppressive effect of PFF on FSH release and synthesis with a less inhibitory effect on LH release mainly at the pituitary level.
Right : To investigate a mode and a site of action of inhibin on FSH and LH secretion, in vivo and in vitro effects of porcine follicular fluid (PFF) on FSH and LH secretion were studied in male rats. PFF administerd i.v. suppressed basal FSH levels for 1-8 hr, but did not alter LH levels. On the contrary, PFF given either 1 or 6 hr in advance, suppressed LH-RH stimulated LH responses as well as LH-RH induced FSH responses. In cultured pituitary cells PFF exerted a partial suppressive effect on LH release. PFF suppressed LH release only under the maximal stimulation with 10^-10 M LH-RH and 0.1mM 3-isobutyl-l-metylxanthine (IBMX) to a lesser degree compared with uniform and greater suppression on FSH release regardless of the experimental conditions. Above results showed a selective suppressive effect of PFF on FSH release and synthesis with a less inhibitory effect on LH release mainly at the pituitary level.

Date of correction: August 31, 2006 Reason for correction: - Correction: CITATION Details: Wrong : 1) Baker, H.M.G., Bremner, W.J., Burger, H.G., de Kretser, D.M., Dulmanis, A., Eddie, L.W., Hudson, B., Keogh, E.J., Lee, V.W.K. & Rennie, G.C. (1976) Testicular control of follicle-stimulating hormone secretion. Recent. Progr. Horm. Res., 32, 429-476.
2) Channing, C.P., Anderson, L.D., Hoover, D.J., Gagliano, P. & Hodgen, G. (1981) Inhibitory effects of porcine follicular fluid on monkey serum FSH levels and follicular maturation. Biol. Reprod., 25, 885-903.
3) de Jong, F.H., Smith, S.D. & van der Molen, H.J. (1979) Bioassay of inhibin-like activity using pituitary cells in vitro. J. Endocr., 80, 91-102.
4) Demoulin, A., Bourguignon, J.P. & Franchimont, P. (1979) “In vitro” inhibition of hypothalamic LH-RH by human inhibin. Acta endocr. (Kbh.), Suppl. 225, 227.
5) Demura, R., Nakamura, S., Komatsu, H., Suzuki, T., Jibiki, K., Odagiri, E., Demura, H. & Shizume, K. (1987a) Short term treatments with prolactin, HMG or testosterone fail to affect testicular inhibin content in rats. Endocr. jap., 34, 139-143.
6) Demura, R., Suzuki, T., Nakamura, S., Komatsu, H., Jibiki, K., Odagiri, E., Demura, H. & Shizume, K. (1987b) Effect of uni- and bilateral cryptorchidism on testicular inhibin and testosterone secretion in rats. Endocr. jap., 34, 911-917.
7) Depaolo, L.V., Wise, P.M., Anderson, L.D., Barraclough, C.A. & Channing, C.P. (1979) Suppression of the pituitary follicle-stimulating hormone secretion during proestrus and estrus in rats by porcine follicular fluid: Possible site of action. Endocrinology, 104, 402-408.
8) Franchimont, P., Chari, S. & Demoulin, A. (1975) Hypothalamus-pituitary-testis interactions. J. Reprod. Fertil., 44, 335-350.
9) Hopkinson, C.R., Daume, E., Sturm, G., Fritze, E., Kaiser, S. & Hirschhauser, C. (1977) Inhibin-like activity of bovine ovarian extracts in male and female rats. J. Reprod. Fertil., 50, 93-96.
10) Huang, S.E. & Miller, W.L. (1984) Porcine ovarian inhibin preparations sensitize cultured ovine gonadotrophs to luteinizing hormone-releasing hormone. Endocrinology, 115, 513-519.
11) Koiter, T.R., van del Schaaf-Verdonk, G.C., Kuiper, H., Pols-Valkhof, N. & Schuiling, G.A. (1983) Control of follicle-stimulating hormome secretion by steroid-free bovine follicular fluid in the ovariectomized rat. J. Endocr., 99, 1-8.
12) Labrié, F., Lagace, L., Drouin, J., Kelly, P.A., Massicotte, J. & Dorrington, J.H. (1978) Interaction between LH-RH, sex steroids and “inhibin” in the control of LH and FSH secretion. In: Endocrine Approach to Male Contraception, edited by V. Hanson, Scriptor, Copenhargen, p. 81.
13) Lagacé, L., Labrie, F., Lorenzen, J., Schwarts, N.B. & Channing, C.P. (1979) Selective inhibitory effect of porcine follicular fluid on follicle stimulating hormone secretion in anterior pituitary cells in culture. Clin. Endocr., 10, 401-405.
14) Lorenzen, J.R., Channing, C.P. & Schwartz, N.B. (1978) Partial characterization of FSH suppressing activity (folliculostatin) in porcine follicular fluid using the metes trous rat as an in vivo bioassay model. Biol. Reprod., 19, 635-640.
15) Lumpkin, M., Negro-Vilar, A., Franchimont, P. & McCann, S. (1981) Evidence for a hypothalamic site of action of inhibin to suppress FSH release. Endocrinology, 108, 1101-1104.
16) Marder, M.L., Channing, C.P. & Schwartz, N.B. (1977) Suppression of serum follicle stimulating hormone in intact and acutely ovariectomized rats by porcine follicular fluid. Endocrinology, 101, 1639-1642.
17) Moodbidri, S.B., Vijayalakshmi, S., Bandivdekar, A.H. & Sheth, A.R. (1981) Inhibition of hypothalamic GnRH synthesis by inhibin. Experientia, 37, 525-526.
18) Rettori, V., Siler-Khodr, T.M., Pauestein, C.J., Smith, C.G. & Asch, R.H. (1982) Effects of porcine follicular fluid on gonadotropin concentrations in rhesus monkeys. J. clin. Endocr., 54, 500-503.
19) Rush, M.E., Ashiru, O.A., Lipner, H., Williams, A.T., McRae, C. & Blake, C.A. (1981) The actions of porcine follicular fluid and estradiol on periovulatory secretion of gonadotropic hormones in rats. Endocrinology, 108, 2316-2323.
20) Schwartz, N.B. & Channing, C.P. (1977) Evidence for ovarian “inhibin” suppression of the secondary rise in serum follicle stimulating hormone levels in proestrous rats by injection of porcine follicular fluid. Proc. nat. Acad. Sci. USA, 74, 5721.
21) Shander, D., Anderson, L.D., Barraclough, C.A. & Channing, C.P. (1980) Interactions of porcine follicular fluid with ovarian steroids and luteinizing hormone-releasing hormone on the secretion of luteinizing hormone and follicle-stimulating hormone by cultured pituitary cells. Endocrinology, 106, 237-242.
22) Shiraishi, S. (1986) Studies on inhibin in human follicular fluid. Acta obstet. gynaec. jap., 38, 741-749. (Japanese)

Right : 1) Baker, H. M. G., Bremner, W. J., Burger, H. G., de Kretser, D. M., Dulmanis, A., Eddie, L. W., Hudson, B., Keogh, E. J. , Lee, V. W. K. & Rennie, G. C. (1976) Testicular control of follicle-stimulating hormone secretion. Recent. Progr. Horm. Res., 32, 429-476.
2) Channing, C. P., Anderson, L. D., Hoover, D. J., Gagliano, P. & Hodgen, O. (1981) Inhibitory effects of porcine follicular fluid on monkey serum FSH levels and follicular maturation. Biol. Reprod., 25, 885-903.
3) de Jong, F. H., Smith, S. D. & van der Molen, H. J. (1979) Bioassay of inhibin-like activity using pituitary cells in vitro. J. Endocr., 80, 91-102.
4) Demoulin, A., Bourguignon, J. P. & Franchimont, P. (1979) “In vitro” inhibition of hypothalamic LH-RH by human inhibin. Acta endocr. (Kbh.), Suppl. 225, 227.
5) Demura, R., Nakamura, S., Komatsu, H., Suzuki, T., Jibiki, K., Odagiri, E., Demura, H. & Shizume, K. (1987a) Short term treatments with prolactin, HMG or testosterone fail to affect testicular inhibin content in rats. Endocr. jap., 34, 139-143.
6) Demura, R., Suzuki, T., Nakamura, S., Komatsu, H., Jibiki, K., Odagiri, E., Demura, H. & Shizume, K. (1987b) Effect of uni- and bilateral cryptorchidism on testicular inhibin and testosterone secretion in rats. Endocr. jap., 34, 911-917.
7) Depaolo, L. V., Wise, P. M., Anderson, L. D., Barraclough, C. A. & Channing, C. P. (1979) Suppression of the pituitary follicle-stimulating hormone secretion during proestrus and estrus in rats by porcine follicular fluid: Possible site of action. Endocrinology, 104, 402-408.
8) Franchimont, P., Chari, S. & Demoulin, A. (1975) Hypothalamus-pituitary-testis interactions. J. Reprod. Fertil., 44, 335-350.
9) Hopkinson, C. R., Daume, E., Sturm, G., Fritze, E., Kaiser, S. & Hirschhauser, C. (1977) Inhibin-like activity of bovine ovarian extracts in male and female rats. J. Reprod. Fertil., 50, 93-96.
10) Huang, S. E. & Miller, W. L. (1984) Porcine ovarian inhibin preparations sensitize cultured ovine gonadotrophs to luteinizing hormone-releasing hormone. Endocrinology, 115, 513-519.
11) Koiter, T. R., van del Schaaf-Verdonk, G. C., Kuiper, H., Pols-Valkhof, N. & Schuiling, G. A. (1983) Control of follicle-stimulating hormome secretion by steroid-free bovine follicular fluid in the ovariectomized rat. J. Endocr., 99, 1-8.
12) Labrié, F., Lagace, L., Drouin, J., Kelly, P. A., Massicotte, J. & Dorrington, J. H. (1978) Interaction between LH-RH, sex steroids and “inhibin” in the control of LH and FSH secretion. In: Endocrine Approach to Male Contraception, edited by V. Hanson, Scriptor, Copenhargen, p. 81.
13) Lagacé, L., Labrie, F., Lorenzen, J., Schwarts, N. B. & Channing, C. P. (1979) Selective inhibitory effect of porcine follicular fluid on follicle stimulating hormone secretion in anterior pituitary cells in culture. Clin. Endocr., 10, 401-405.
14) Lorenzen, J. R., Channing, C. P. & Schwartz, N. B. (1978) Partial characterization of FSH suppressing activity (folliculostatin) in porcine follicular fluid using the metestrous rat as an in vivo bioassay model. Biol. Reprod., 19, 635-640.
15) Lumpkin, M., Negro-Vilar, A., Franchimont, P. & McCann, S. (1981) Evidence for a hypothalamic site of action of inhibin to suppress FSH release. Endocrinology, 108, 1101-1104.
16) Marder, M. L., Channing, C. F. & Schwartz, N. B. (1977) Suppression of serum follicle stimulating hormone in intact and acutely ovariectomized rats by porcine follicular fluid. Endocrinology, 101, 1639-1642.
17) Moodbidri, S. B., Vijayalakshmi, S., Bandivdekar, A. H. & Sheth, A. R. (1981) Inhibition of hypothalamic GnRH synthesis by inhibin. Experientia, 37, 525-526.
18) Rettori, V., Siler-Khodr, T. M., Pauestein, C. J., Smith, C. G. & Asch, R. H. (1982) Effects of porcine follicular fluid on gonadotropin concentrations in rhesus monkeys. J. clin. Endocr., 54, 500-503.
19) Rush, M. E., Ashiru, O. A., Lipner, H., Williams, A. T., McRae, C. & Blake, C. A. (1981) The actions of porcine follicular fluid and estradiol on periovulatory secretion of gonadotropic hormones in rats. Endocrinology, 108, 2316-2323.
20) Schwartz, N. B. & Channing, C. P. (1977) Evidence for ovarian “inhibin” suppression of the secondary rise in serum follicle stimulating hormone levels in proestrous rats by injection of porcine follicular fluid. Proc. nat. Acad. Sci. USA, 74, 5721.
21) Shander, D., Anderson, L. D., Barraclough, C. A. & Channing, C. P. (1980) Interactions of porcine follicular fluid with ovarian steroids and luteinizing hormone-releasing hormone on the secretion of luteinizing hormone and follicle-stimulating hormone by cultured pituitary cells. Endocrinology, 106, 237-242.
22) Shiraishi, S. (1986) Studies on inhibin in human follicular fluid. Acta obstet. gynaec. jap., 38, 741-749. (Japanese)

Date of correction: August 31, 2006 Reason for correction: - Correction: PDF FILE Details: -

Date of correction: August 31, 2006 Reason for correction: - Correction: ABSTRACT Details: Wrong : To investigate a mode and a site of action of inhibin on FSH and LH secretion, in vivo and in vitro effects of porcine follicular fluid (PFF) on FSH and LH secretion were studied in male rats. PFF administerd i.v. suppressed basal FSH levels for 1-8 hr, but did not alter LH levels. On the contrary, PFF given either 1 or 6 hr in advance, suppressed LH-RH stimulated LH responses as well as LH-RH induced FSH responses. In cultured pituitary cells PFF exerted a partial suppressive effect on LH release. PFF suppressed LH release only under the maximal stimulation with 10^-10 M LH-RH and 0.1mM 3-isobutyl-l-metylxanthine (IBMX) to a lesser degree compared with uniform and greater suppression on FSH release regardless of the experimental conditions. Above results showed a selective suppressive effect of PFF on FSH release and synthesis with a less inhibitory effect on LH release mainly at the pituitary level.
Right : To investigate a mode and a site of action of inhibin on FSH and LH secretion, in vivo and in vitro effects of porcine follicular fluid (PFF) on FSH and LH secretion were studied in male rats. PFF administerd i.v. suppressed basal FSH levels for 1-8 hr, but did not alter LH levels. On the contrary, PFF given either 1 or 6 hr in advance, suppressed LH-RH stimulated LH responses as well as LH-RH induced FSH responses. In cultured pituitary cells PFF exerted a partial suppressive effect on LH release. PFF suppressed LH release only under the maximal stimulation with 10^-10 M LH-RH and 0.1mM 3-isobutyl-1-metylxanthine (IBMX) to a lesser degree compared with uniform and greater suppression on FSH release regardless of the experimental conditions. Above results showed a selective suppressive effect of PFF on FSH release and synthesis with a less inhibitory effect on LH release mainly at the pituitary level.

Date of correction: August 31, 2006 Reason for correction: - Correction: CITATION Details: Wrong : 1) Baker, H. M. G., Bremner, W. J., Burger, H. G., de Kretser, D. M., Dulmanis, A., Eddie, L. W., Hudson, B., Keogh, E. J. , Lee, V. W. K. & Rennie, G. C. (1976) Testicular control of follicle-stimulating hormone secretion. Recent. Progr. Horm. Res., 32, 429-476.
2) Channing, C. P., Anderson, L. D., Hoover, D. J., Gagliano, P. & Hodgen, O. (1981) Inhibitory effects of porcine follicular fluid on monkey serum FSH levels and follicular maturation. Biol. Reprod., 25, 885-903.
3) de Jong, F. H., Smith, S. D. & van der Molen, H. J. (1979) Bioassay of inhibin-like activity using pituitary cells in vitro. J. Endocr., 80, 91-102.
4) Demoulin, A., Bourguignon, J. P. & Franchimont, P. (1979) “In vitro” inhibition of hypothalamic LH-RH by human inhibin. Acta endocr. (Kbh.), Suppl. 225, 227.
5) Demura, R., Nakamura, S., Komatsu, H., Suzuki, T., Jibiki, K., Odagiri, E., Demura, H. & Shizume, K. (1987a) Short term treatments with prolactin, HMG or testosterone fail to affect testicular inhibin content in rats. Endocr. jap., 34, 139-143.
6) Demura, R., Suzuki, T., Nakamura, S., Komatsu, H., Jibiki, K., Odagiri, E., Demura, H. & Shizume, K. (1987b) Effect of uni- and bilateral cryptorchidism on testicular inhibin and testosterone secretion in rats. Endocr. jap., 34, 911-917.
7) Depaolo, L. V., Wise, P. M., Anderson, L. D., Barraclough, C. A. & Channing, C. P. (1979) Suppression of the pituitary follicle-stimulating hormone secretion during proestrus and estrus in rats by porcine follicular fluid: Possible site of action. Endocrinology, 104, 402-408.
8) Franchimont, P., Chari, S. & Demoulin, A. (1975) Hypothalamus-pituitary-testis interactions. J. Reprod. Fertil., 44, 335-350.
9) Hopkinson, C. R., Daume, E., Sturm, G., Fritze, E., Kaiser, S. & Hirschhauser, C. (1977) Inhibin-like activity of bovine ovarian extracts in male and female rats. J. Reprod. Fertil., 50, 93-96.
10) Huang, S. E. & Miller, W. L. (1984) Porcine ovarian inhibin preparations sensitize cultured ovine gonadotrophs to luteinizing hormone-releasing hormone. Endocrinology, 115, 513-519.
11) Koiter, T. R., van del Schaaf-Verdonk, G. C., Kuiper, H., Pols-Valkhof, N. & Schuiling, G. A. (1983) Control of follicle-stimulating hormome secretion by steroid-free bovine follicular fluid in the ovariectomized rat. J. Endocr., 99, 1-8.
12) Labrié, F., Lagace, L., Drouin, J., Kelly, P. A., Massicotte, J. & Dorrington, J. H. (1978) Interaction between LH-RH, sex steroids and “inhibin” in the control of LH and FSH secretion. In: Endocrine Approach to Male Contraception, edited by V. Hanson, Scriptor, Copenhargen, p. 81.
13) Lagacé, L., Labrie, F., Lorenzen, J., Schwarts, N. B. & Channing, C. P. (1979) Selective inhibitory effect of porcine follicular fluid on follicle stimulating hormone secretion in anterior pituitary cells in culture. Clin. Endocr., 10, 401-405.
14) Lorenzen, J. R., Channing, C. P. & Schwartz, N. B. (1978) Partial characterization of FSH suppressing activity (folliculostatin) in porcine follicular fluid using the metestrous rat as an in vivo bioassay model. Biol. Reprod., 19, 635-640.
15) Lumpkin, M., Negro-Vilar, A., Franchimont, P. & McCann, S. (1981) Evidence for a hypothalamic site of action of inhibin to suppress FSH release. Endocrinology, 108, 1101-1104.
16) Marder, M. L., Channing, C. F. & Schwartz, N. B. (1977) Suppression of serum follicle stimulating hormone in intact and acutely ovariectomized rats by porcine follicular fluid. Endocrinology, 101, 1639-1642.
17) Moodbidri, S. B., Vijayalakshmi, S., Bandivdekar, A. H. & Sheth, A. R. (1981) Inhibition of hypothalamic GnRH synthesis by inhibin. Experientia, 37, 525-526.
18) Rettori, V., Siler-Khodr, T. M., Pauestein, C. J., Smith, C. G. & Asch, R. H. (1982) Effects of porcine follicular fluid on gonadotropin concentrations in rhesus monkeys. J. clin. Endocr., 54, 500-503.
19) Rush, M. E., Ashiru, O. A., Lipner, H., Williams, A. T., McRae, C. & Blake, C. A. (1981) The actions of porcine follicular fluid and estradiol on periovulatory secretion of gonadotropic hormones in rats. Endocrinology, 108, 2316-2323.
20) Schwartz, N. B. & Channing, C. P. (1977) Evidence for ovarian “inhibin” suppression of the secondary rise in serum follicle stimulating hormone levels in proestrous rats by injection of porcine follicular fluid. Proc. nat. Acad. Sci. USA, 74, 5721.
21) Shander, D., Anderson, L. D., Barraclough, C. A. & Channing, C. P. (1980) Interactions of porcine follicular fluid with ovarian steroids and luteinizing hormone-releasing hormone on the secretion of luteinizing hormone and follicle-stimulating hormone by cultured pituitary cells. Endocrinology, 106, 237-242.
22) Shiraishi, S. (1986) Studies on inhibin in human follicular fluid. Acta obstet. gynaec. jap., 38, 741-749. (Japanese)

Right : 1) Baker, H. M. G., Bremner, W. J., Burger, H. G., de Kretser, D. M., Dulmanis, A., Eddie, L. W., Hudson, B., Keogh, E. J., Lee, V. W. K. & Rennie, G. C. (1976) Testicular control of follicle-stimulating hormone secretion. Recent. Progr. Horm. Res., 32, 429-476.
2) Channing, C. P., Anderson, L. D., Hoover, D. J., Gagliano, P. & Hodgen, G. (1981) Inhibitory effects of porcine follicular fluid on monkey serum FSH levels and follicular maturation. Biol. Reprod., 25, 885-903.
3) de Jong, F. H., Smith, S. D. & van der Molen, H. J. (1979) Bioassay of inhibin-like activity using pituitary cells in vitro. J. Endocr., 80, 91-102.
4) Demoulin, A., Bourguignon, J. P. & Franchimont, P. (1979) “In vitro” inhibition of hypothalamic LH-RH by human inhibin. Acta endocr. (Kbh.), Suppl. 225, 227.
5) Demura, R., Nakamura, S., Komatsu, H., Suzuki, T., Jibiki, K., Odagiri, E., Demura, H. & Shizume, K. (1987a) Short term treatments with prolactin, HMG or testosterone fail to affect testicular inhibin content in rats. Endocr. jap., 34, 139-143.
6) Demura, R., Suzuki, T., Nakamura, S., Komatsu, H., Jibiki, K., Odagiri, E., Demura, H. & Shizume, K. (1987b) Effect of uni- and bilateral cryptorchidism on testicular inhibin and testosterone secretion in rats. Endocr. jap., 34, 911-917.
7) Depaolo, L. V., Wise, P. M., Anderson, L. D., Barraclough, C. A. & Channing, C. P. (1979) Suppression of the pituitary follicle-stimulating hormone secretion during proestrus and estrus in rats by porcine follicular fluid: Possible site of action. Endocrinology, 104, 402-408.
8) Franchimont, P., Chari, S. & Demoulin, A. (1975) Hypothalamus-pituitary-testis interactions. J. Reprod. Fertil., 44, 335-350.
9) Hopkinson, C. R., Daume, E., Sturm, G., Fritze, E., Kaiser, S. & Hirschhauser, C. (1977) Inhibin-like activity of bovine ovarian extracts in male and female rats. J. Reprod. Fertil., 50, 93-96.
10) Huang, S. E. & Miller, W. L. (1984) Porcine ovarian inhibin preparations sensitize cultured ovine gonadotrophs to luteinizing hormone-releasing hormone. Endocrinology, 115, 513-519.
11) Koiter, T. R., van del Schaaf-Verdonk, G. C., Kuiper, H., Pols-Valkhof, N. & Schuiling, G. A. (1983) Control of follicle-stimulating hormome secretion by steroid-free bovine follicular fluid in the ovariectomized rat. J. Endocr., 99, 1-8.
12) Labrié, F., Lagace, L., Drouin, J., Kelly, P. A., Massicotte, J. & Dorrington, J. H. (1978) Interaction between LH-RH, sex steroids and “inhibin” in the control of LH and FSH secretion. In: Endocrine Approach to Male Contraception, edited by V. Hanson, Scriptor, Copenhargen, p. 81.
13) Lagacé, L., Labrie, F., Lorenzen, J., Schwarts, N. B. & Channing, C. P. (1979) Selective inhibitory effect of porcine follicular fluid on follicle stimulating hormone secretion in anterior pituitary cells in culture. Clin. Endocr., 10, 401-405.
14) Lorenzen, J. R., Channing, C. P. & Schwartz, N. B. (1978) Partial characterization of FSH suppressing activity (folliculostatin) in porcine follicular fluid using the metestrous rat as an in vivo bioassay model. Biol. Reprod., 19, 635-640.
15) Lumpkin, M., Negro-Vilar, A., Franchimont, P. & McCann, S. (1981) Evidence for a hypothalamic site of action of inhibin to suppress FSH release. Endocrinology, 108, 1101-1104.
16) Marder, M. L., Channing, C. P. & Schwartz, N. B. (1977) Suppression of serum follicle stimulating hormone in intact and acutely ovariectomized rats by porcine follicular fluid. Endocrinology, 101, 1639-1642.
17) Moodbidri, S. B., Vijayalakshmi, S., Bandivdekar, A. H. & Sheth, A. R. (1981) Inhibition of hypothalamic GnRH synthesis by inhibin. Experientia, 37, 525-526.
18) Rettori, V., Siler-Khodr, T. M., Pauestein, C. J., Smith, C. G. & Asch, R. H. (1982) Effects of porcine follicular fluid on gonadotropin concentrations in rhesus monkeys. J. clin. Endocr., 54, 500-503.
19) Rush, M. E., Ashiru, O. A., Lipner, H., Williams, A. T., McRae, C. & Blake, C. A. (1981) The actions of porcine follicular fluid and estradiol on periovulatory secretion of gonadotropic hormones in rats. Endocrinology, 108, 2316-2323.
20) Schwartz, N. B. & Channing, C. P. (1977) Evidence for ovarian “inhibin” suppression of the secondary rise in serum follicle stimulating hormone levels in proestrous rats by injection of porcine follicular fluid. Proc. nat. Acad. Sci. USA, 74, 5721.
21) Shander, D., Anderson, L. D., Barraclough, C. A. & Channing, C. P. (1980) Interactions of porcine follicular fluid with ovarian steroids and luteinizing hormone-releasing hormone on the secretion of luteinizing hormone and follicle-stimulating hormone by cultured pituitary cells. Endocrinology, 106, 237-242.
22) Shiraishi, S. (1986) Studies on inhibin in human follicular fluid. Acta obstet. gynaec. jap., 38, 741-749. (Japanese)

Date of correction: August 31, 2006 Reason for correction: - Correction: PDF FILE Details: -

Abstract

To investigate a mode and a site of action of inhibin on FSH and LH secretion, in vivo and in vitro effects of porcine follicular fluid (PFF) on FSH and LH secretion were studied in male rats. PFF administerd i.v. suppressed basal FSH levels for 1-8 hr, but did not alter LH levels. On the contrary, PFF given either 1 or 6 hr in advance, suppressed LH-RH stimulated LH responses as well as LH-RH induced FSH responses. In cultured pituitary cells PFF exerted a partial suppressive effect on LH release. PFF suppressed LH release only under the maximal stimulation with 10^-10 M LH-RH and 0.1mM 3-isobutyl-1-metylxanthine (IBMX) to a lesser degree compared with uniform and greater suppression on FSH release regardless of the experimental conditions. Above results showed a selective suppressive effect of PFF on FSH release and synthesis with a less inhibitory effect on LH release mainly at the pituitary level.

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