Establishment and Characterization of an Epstein-Barr Virus Negative B-Cell Lymphoma Cell Line and Successful Heterotransplantation

YOSHIHIRO NOZAWA; MASAFUMI ABE; HARUKI WAKASA; HITOSHI OHNO; SHIROU FUKUHARA; TOSHIBUMI KINOSHITA; TOYOROU OSATO

doi:10.1620/tjem.156.319

YOSHIHIRO NOZAWA, MASAFUMI ABE, HARUKI WAKASA, HITOSHI OHNO, SHIROU FUKUHARA, TOSHIBUMI KINOSHITA, TOYOROU OSATO

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Keywords: Epstein-Barr virus nuclear antigen (EBNA), B-cell lymphoma cell line, heter-otransplantation

JOURNAL FREE ACCESS

1988 Volume 156 Issue 4 Pages 319-330

DOI https://doi.org/10.1620/tjem.156.319

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Published: 1988 Received: August 08, 1988 Available on J-STAGE: August 31, 2006 Accepted: October 07, 1988 Advance online publication: - Revised: -
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Date of correction: August 31, 2006 Reason for correction: - Correction: ABSTRACT Details: Wrong : NOZAWA, Y., ABE, M., WAKASA, H., OHNO, H., FUKUHARA, S., KINOSHITA, T. and OSATO, T. Establishment and Characterization of an Epstein-Barr Virus Negative B-Cell Lymphoma Cell Line and Successful Heterotransplantation. Tohoku J. exp. Med., 1988, 156 (4), 319-330-An EBV-negative B-cell lymphoma cell line designated as HBL-1 was established from the pleural effusion of a patient with malignant lymphoma, diffuse, large cell. Surface marker studies revealed that HBL-1 cell possessed the same phenotypes of a mature B-cell as the original lymphoma cell from pleural effusion. HBL-1 closely resembled morphologically to the original lymphoma cells observed in the lymph node biopsy and pleural effusion. Chromosome analysis revealed that HBL-1 cells showed chromosomal aberrations of a 14q+marker chromosome as the result of a translocation between chromosomes 14 and 16. Heterotransplantation to subcutaneous tissue of an athymic nude mouse has succeeded, but intraperitoneal heterotransplantation has failed. Tumors were localized in the subcutaneous tissues and no metastasis was found. This newly established cell line may facilitate immunological and oncogenic studies for human B-cell lymphomas.
Right : An EBV-negative B-cell lymphoma cell line designated as HBL-1 was established from the pleural effusion of a patient with malignant lymphoma, diffuse, large cell. Surface marker studies revealed that HBL-1 cell possessed the same phenotypes of a mature B-cell as the original lymphoma cell from pleural effusion. HBL-1 closely resembled morphologically to the original lymphoma cells observed in the lymph node biopsy and pleural effusion. Chromosome analysis revealed that HBL-1 cells showed chromosomal aberrations of a 14q + marker chromosome as the result of a translocation between chromosomes 14 and 16. Heterotransplantation to subcutaneous tissue of an athymic nude mouse has succeeded, but intraperitoneal heterotransplantation has failed. Tumors were localized in the subcutaneous tissues and no metastasis was found. This newly established cell line may facilitate immunological and oncogenic studies for human B-cell lymphomas.

Date of correction: August 31, 2006 Reason for correction: - Correction: KEYWORD Details: Wrong : Epstein-Barr virus nuclear antigen (EBNA), B-cell lymphoma cell line, heterotransplantation
Right : Epstein-Barr virus nuclear antigen (EBNA), B-cell lymphoma cell line, heter-otransplantation

Date of correction: August 31, 2006 Reason for correction: - Correction: CITATION Details: Wrong : 1) Abe, M., Wakasa, H., Mori, N. & Kojima, M. (1983) Gamma-heavy chain disease.Ultrastructural and immunohistochemical study. Acta path. jap., 33, 689-700.
2) Dillman, R.O., Handley, H.H. & Royston, I. (1982) Establishment and characterizationof an Epstein-Barr virus-negative lymphoma B-cell line from a patient with adiffuse large cell lymphoma. Cancer Res., 42, 1368-1373.
3) Diehl, V., Krause, P., Hellriegel, K.P., Busche, M., Schedel, I. & Laskewitz, E. (1977)Lymphoid cell lines: In vitro cell markers in correlation to tumorigenicity in nudemice. Hemat. Blood Transfus., 20, 289-296.
4) Epstein, A.L. & Kaplan, H.S. (1974) Biology of the human malignant lymphomas. I. Establishment in continuous cell culture and heterotransplantation of diffusehistiocytic lymphomas. Cancer, 34, 1851-1872.
5) Epstein, A.L., Levy, R., Kim, H., Henle, W., Henle, G. & Kaplan, H.S. (1978) Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer, 42, 2379-2391.
6) Fukuhara, S., Ueshima, Y., Kita, K. & Uchino, H. (1984) 14q⁺ marker-positive lymphoid cancer and its subclasses. Acta haemat. jap., 47, 1579-1590.
7) Hayashi, Y., Matsumura, Y., Nishihira, T., Watanabe, I., Ohi, R., Kasai, M., Kumagai, K. & Kamada, N. (1980) Burkitt's lymphoma cell line bearing surface IgA and negative for nuclear antigen of Epstein-Barr virus (EBNA). Jap. J. exp. Med., 50, 423-434.
8) Ikeuchi, T. (1984) Inhibitory effect of ethidium bromide on mitotic chromosome condensation and its application to high-resolution chromosome banding. Cytogenet. Cell Genet., 38, 56-61.
9) Kaplan, H.S., Goodenow, R.S., Gartner, S. & Bieber, M.M. (1979) Biology andvirology of the human malignant lymphomas. Cancer, 43, 1-24.
10) Klein, G., Lindahl, T., Jondal, M., Leibold, W., Menezes, J., Nilsson, K. & Sundström, C. (1974) Continuous lymphoid cell lines with characteristics of B cell (bone-marrow derived), lacking the Epstein-Barr virus genome and derived from three human lymphomas. Proc. nat. Acad. Sci. USA, 71, 3283-3286.
11) Magrath, I.T., Pizzo, P.A., Whang-Peng, J., Douglass, E.C., Alabaster, O., Gerber, P., Freeman, C.B. & Novikovs, L. (1980a) Characterization of lymphoma-derived cell lines. Comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical cytogenetic, and growth characteristics. J. nat. Cancer Inst., 64, 465-476.
12) Magrath, I.T., Freeman, C.B., Pizzo, P.A., Gadek, J., Jaffe, E., Santaella, M., Hammer, C., Frank, M., Reaman, G. & Novikovs, L. (1980b) Characterization of lymphomaderived cell lines: Comparison of cell lines positive and negative for Epstein-Barr virus nuclear antiges. II. Surface marker. J. nat. Cancer Inst., 64, 477-483.
13) Nilsson, K. (1977) Established cell lines as tools in the study of human lymphoma and myeloma cell characteristics. Hemat. Blood Transfu., 20, 253-264.
14) Nilsson, K. & Protén, J. (1975) Classification and biological nature of established human hematopoietic cell lines. Int. J. Cancer, 15, 321-341.
15) Nilsson, K. & Sundström, C. (1974) Establishment and characteristics of two unique cell lines from patients with lymphosarcoma. Int. J. Cancer, 13, 808-823.
16) Nilsson, K., Giovanella, B.C., Stehlin, J.S. & Klein, G. (1977) Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int. J. Cancer, 19, 337-344.
17) Nilsson, K., Klareskog, L., Ralph, P., Sundstrom, C. & Zech, L. (1983) Phenotypic and cytogenetic characteristics of a new Epstein-Barn virus negative cell line (SKW4) derived from a B-cell lymphoma. Hemat. Oncol., 1, 277-295.
18) Nishikiori, M., Hansen, H., Jhanwer, S., Freid, J., Sordillo, P., Koziner, B., Lloyd, K. & Clarkson, B. (1984) Establishment of a neartetraploid B-cell lymphoma line with duplication of the 8; 14 translocation. Cancer Genet. Cytogenet., 12, 39-50.
19) Ohno, H., Doi, S., Fukuhara, S., Nishikiori, M., Uchino, H. & Fujii, H. (1985) A newly established human lymphoma cell line, FL-18, carrying a 14; 18 translocation. Jap. J. Cancer Res. (Gann), 76, 563-566.
20) Povlsen, C.O., Fialkow, P.J., Klein, E., Klein, G., Rygaard, J. & Wiener, F. (1973) Growth and antigenic properties of a biopsy derived Burkitt's lymphoma in thymus-less (nude) mice. Int. J. Cancer, 11, 30-39.
21) Pulvertaft, R.J. (1964) Cytology of Burkitt's tumor (African lymphoma). Lancet, 1, 238-240.
22) Report of the Standing Committee on Cuman Cytogenetic Nomenclature. An International System for Human Cytogenetic Nomenclature-High-Resolution Banding. (1981) Cytogenet. Cell Genet., 5-32.
23) Southern, E.M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. molec. Biol., 98, 503-517.
24) Sundström, C. & Nilsson, K. (1978) Human malignant lymphomas in vitro. Characterization of biopsy cells and establishment of permanent cell lines. Acta path. microbiol. scand. Sect. A, 86, 173-184.
25) Suzuki, M. & Hinuma, Y. (1974) Evaluation of Epstein-Barn virus-assoicated nuclear antigen with various human cell lines. Int. J. Cancer, 14, 753-761.
26) The Non-Hodgkin's Lymphoma Pathologic Classification Project (1982) National Cancer Institution sponsored study of classifications of non-Hodgkin's lymphomas: Summary and description of a working formulation for clinical usage. Cancer, 49, 2112-2135.
27) Watanabe, S., Kuroki, M., Sato, Y., Shimosato, Y. & Hasegawa, T. (1980a) The establishment of a cell line (NH-AR) from a human nodular lymphoma and a comparison with lymphoblastoid cell line. Cancer, 46, 2438-2445.
28) Watanabe, S., Shimosato, Y., Kuroki, M., Sato, Y. & Nakajima, T. (1980b) Transplantability of human lymphoid cell line, lymphoma and leukemia in splenectomized and/or irradiated nude mice. Cancer Res., 40, 2588-2595.

Right : 1) Abe, M., Wakasa, H., Mori, N. & Kojima, M. (1983) Gamma-heavy chain disease. Ultrastructural and immunohistochemical study. Acta path. jap., 33, 689-700.
2) Dillman, R. O., Handley, H. H. & Royston, I. (1982) Establishment and characterization of an Epstein-Barr virus-negative lymphoma B-cell line from a patient with a diffuse large cell lymphoma. Cancer Res., 42, 1368-1373.
3) Diehl, V., Krause, P., Hellriegel, K. P., Busche, M., Schedel, I. & Laskewitz, E. (1977) Lymphoid cell lines: In vitro cell markers in correlation to tumorigenicity in nude mice. Hemat. Blood Transfus., 20, 289-296.
4) Epstein, A. L. & Kaplan, H. S. (1974) Biology of the human malignant lymphomas. I. Establishment in continuous cell culture and heterotransplantation of diffuse histiocytic lymphomas. Cancer, 34, 1851-1872.
5) Epstein, A. L., Levy, R., Kim, H., Henle, W., Henle, G. & Kaplan, H. S. (1978) Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer, 42, 2379-2391.
6) Fukuhara, S., Ueshima, Y., Kita, K. & Uchino, H. (1984) 14q⁺ marker-positive lymphoid cancer and its subclasses. Acta haemat. jap., 47, 1579-1590.
7) Hayashi, Y., Matsumura, Y., Nishihira, T., Watanabe, I., Ohi, R., Kasai, M., Kumagai, K. & Kamada, N. (1980) Burkitt's lymphoma cell line bearing surface IgA and negative for nuclear antigen of Epstein-Barr virus (EBNA). Jap. J. exp. Med., 50, 423-434.
8) Ikeuchi, T. (1984) Inhibitory effect of ethidium bromide on mitotic chromosome condensation and its application to high-resolution chromosome banding. Cytogenet. Cell Genet., 38, 56-61.
9) Kaplan, H. S., Goodenow, R. S., Gartner, S. & Bieber, M. M. (1979) Biology and virology of the human malignant lymphomas. Cancer, 43, 1-24.
10) Klein, G., Lindahl, T., Jondal, M., Leibold, W., Menezes, J., Nilsson, K. & Sundström, C. (1974) Continuous lymphoid cell lines with characteristics of B cell (bone-marrow derived.), lacking the Epstein-Barr virus genome and derived from three human lymphomas. Proc. nat. Acad. Sci. USA, 71, 3283-3286.
11) Magrath, I. T., Pizzo, P. A., Whang-Peng, J., Douglass, E. C., Alabaster, O., Gerber, P., Freeman, C. B. & Novikovs, L. (1980a) Characterization of lymphoma-derived cell lines. Comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical cytogenetic, and growth characteristics. J. nat. Cancer Inst., 64, 465-476.
12) Magrath, I. T., Freeman, C. B., Pizzo, P. A., Gadek, J., Jaffe, E., Santaella, M., Hammer, C., Frank, M., Reaman, G. & Novikovs, L. (1980b) Characterization of lymphoma derived cell lines: Comparison of cell lines positive and negative for Epstein-Barr virus nuclear antiges. II. Surface marker. J. nat. Cancer Inst., 64, 477-483.
13) Nilsson, K. (1977) Established cell lines as tools in the study of human lymphoma and myeloma cell characteristics. Hemat. Blood Transfu., 20, 253-264.
14) Nilsson, K. & Protén, J. (1975) Classification and biological nature of established human hematopoietic cell lines. Int. J. Cancer, 15, 321-341.
15) Nilsson, K. & Sundström, C. (1974) Establishment and characteristics of two unique cell lines from patients with lymphosarcoma. Int. J. Cancer, 13, 808-823.
16) Nilsson, K., Giovanella, B. C., Stehlin, J. S. & Klein, G. (1977) Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int. J. Cancer, 19, 337-344.
17) Nilsson, K., Klareskog, L., Ralph, P., Sundström, C. & Zech, L. (1983) Phenotypic and cytogenetic characteristics of a new Epstein-Barr virus negative cell line (SKW4) derived from a B-cell lymphoma. Hemat. Oncol., 1, 277-295.
18) Nishikiori, M., Hansen, H., Jhanwer, S., Freid, J., Sordillo, P., Koziner, B., Lloyd, K. & Clarkson, B. (1984) Establishment of a neartetraploid B-cell lymphoma line with duplication of the 8; 14 translocation. Cancer Genet. Cytogenet., 12, 39-50.
19) Ohno, H., Doi, S., Fukuhara, S., Nishikiori, M., Uchino, H. & Fujii, H. (1985) A newly established human lymphoma cell line, FL-18, carrying a 14; 18 translocation. Jap. J. Cancer Res. (Gann), 76, 563-566.
20) Povlsen, C. O., Fialkow, P. J., Klein, E., Klein, G., Rygaard, J. & Wiener, F. (1973) Growth and antigenic properties of a biopsy derived Burkitt's lymphoma in thymusless (nude) mice. Int. J. Cancer, 11, 30-39.
21) Pulvertaft, R. J. (1964) Cytology of Burkitt's tumor (African lymphoma). Lancet, 1, 238-240.
22) Report of the Standing Committee on Cuman Cytogenetic Nomenclature. An International System for Human Cytogenetic Nomenclature-High-Resolution Banding. (1981) Cytogenet. Cell Genet., 5-32.
23) Southern, E. M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. molec. Biol., 98, 503-517.
24) Sundström, C. & Nilsson, K. (1978) Human malignant lymphomas in vitro. Characterization of biopsy cells and establishment of permanent cell lines. Acta path. microbiol. scand. Sect. A, 86, 173-184.
25) Suzuki, M. & Hinuma, Y. (1974) Evaluation of Epstein-Barr virus-assoicated nuclear antigen with various human cell lines. Int. J. Cancer, 14, 753-761.
26) The Non-Hodgkin's Lymphoma Pathologic Classification Project (1982) National Cancer Institution sponsored study of classifications of non-Hodgkin's lymphomas: Summary and description of a working formulation for clinical usage. Cancer, 49, 2112-2135.
27) Watanabe, S., Kuroki, M., Sato, Y., Shimosato, Y. & Hasegawa, T. (1980a) The establishment of a cell line (NH-AR) from a human nodular lymphoma and a comparison with lymphoblastoid cell line. Cancer, 46, 2438-2445.
28) Watanabe, S., Shimosato, Y., Kuroki, M., Sato, Y. & Nakajima, T. (1980b) Transplantability of human lymphoid cell line, lymphoma and leukemia in splenectomized and/or irradiated nude mice. Cancer Res., 40, 2588-2595.

Date of correction: August 31, 2006 Reason for correction: - Correction: PDF FILE Details: -

Abstract

An EBV-negative B-cell lymphoma cell line designated as HBL-1 was established from the pleural effusion of a patient with malignant lymphoma, diffuse, large cell. Surface marker studies revealed that HBL-1 cell possessed the same phenotypes of a mature B-cell as the original lymphoma cell from pleural effusion. HBL-1 closely resembled morphologically to the original lymphoma cells observed in the lymph node biopsy and pleural effusion. Chromosome analysis revealed that HBL-1 cells showed chromosomal aberrations of a 14q + marker chromosome as the result of a translocation between chromosomes 14 and 16. Heterotransplantation to subcutaneous tissue of an athymic nude mouse has succeeded, but intraperitoneal heterotransplantation has failed. Tumors were localized in the subcutaneous tissues and no metastasis was found. This newly established cell line may facilitate immunological and oncogenic studies for human B-cell lymphomas.

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Correction information

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