Abstract
A prolongation of the shortest peroxidase stain-time of blood leu-cocytes (=neutrophiles) is, as already reported, a sign of B-avitaminosis. Mothers with Arakawa-negative milk and infants nursed with such a milk show generally the prolongation in question. On an administration of vitamin B the prolongation is restored to normal. The normal shortest peroxidase stain-time is 2'' with the “old technique” (using Sato and Sekiya's original Copper Peroxidase Method) and 4'' with the “new technique” (using its modification, the Tohoku Pediatric Method). In the present experiment the speed of the restoration weas investigated with the “new technique” aswell as the “old technique.”
The results of the present experiment are:
1. The “new technique” shows the restoration of the prolongation to normal more exactly. Or, when the restoration appears completed with the “old technique”, the “new one” shows that it is still incomplete. For instance, when the stain-time has become to 2''-or to normal-with the “old technique”, the stain-time of 7'' has become only to 5'' (instead of 4''-the normal) with the “new technique.” The change is thus shown more exactly.
2. Examined with the “new technique” (or the shortest peroxidase stain method using the Tohoku Pediatric Method), the prolongation of the shortest peroxidase stain-time of blood leucocytes will be restored soon to normal due to the administration of vitamin B. But the time up to the restoration is longer than examined with “old technique”, because the “new technique” shows the result more precisely.
3. The present work confirms further that a prolongation of the shortest peroxidase stain-time of blood leucocytes is a sign of avitaminosis B, and that an administration of vitamin B restores the prolongation to normal.
