1956 Volume 64 Issue 1 Pages 39-50
1. Livers form persons belonging to Group B were fractioned to isolate the Group B lipid by a procedure different from the one which had been previously applied in this Institute.
2. Of the preparations, one was Group B active, though weak, but the others obtained in a similar manner were group-inactive. One of the latter was compared chemically and physically with the former. i) They both showed a single boundary after electrophoresis as well as after ultracentrifugation. ii) They both contained chondrosamine, galactose, glucose, a fatty acid or acids, ethanolamine, glycine, serine, aspartic and glutamic acid and glycerophosphoric acid. iii) They analyzed similar on several constituents. Thus they showed no difference from each other in chemical respects so far as examined, and resembled the preparation of Masamune et al. of Group A lipid from the same source as well, suggesting the homogeneity of the electrophoretically pure preparations of group lipids not only from human liver but also from other tissues. iv) The two preparations, however, differed from each other regarding axial ratio of molecule and molecular weight.
3. Group B potency was scarcely elevated or lost during preparation of the group lipid, nevertheless, the products showed no Group A nor O activity, that is, no group conversion was recognized contrary to the case of group lipids from hog and human lungs. The mechanism involved is discussed, resting on the view of Masamune on group interconversion in vitro.
Through the Grant Committee for Scientific Researches, the Ministry of Education gave a grant in aid to us. H. Masamune.