Nucleic Acids
X. Chemical Comparison between DNAs of Human Placenta and of Human Spleen Third Report: Schiff Reaction of the DNAs during the Course of Acid Hydrolysis
1957 年 65 巻 2-3 号 p. 287-291
詳細
1957 年 65 巻 2-3 号 p. 287-291
1. Schiff reaction has been made up into a quantitative-analytical method for determination of deoxyribose with free carbonyl in degraded DNAs.
2. Occurrence of the Schiff-positive residue was followed up during mild acid hydrolyses of DNAs from human placenta and spleen.
3. The Schiff-positive residue was produced more quickly from the placental DNA than from the splenic DNA, in other words, purines in the placental DNA are less stably linked than those in the splenic DNA. After reaching the maximum, the intensities of the reaction of the DNAs were nearly the same within 16 hours and then decreased gradually. The maximum values equaled each other.
The expenses of this work were defrayed by a grant from the Ministry of Health and Welfare, which is gratefully acknowledged. H. Masamune.
