Chemistry and Biology of Lipids
XXVI. Fractionation of Group Lipid Mixtures of Pig Stomach and Pig Lung by Precipitin Reaction
1957 Volume 66 Issue 3-4 Pages 263-279
Details
1957 Volume 66 Issue 3-4 Pages 263-279
1. A group lipid mixture AO (Lipids AO) was preparea from pig stomachs and a mixture BO (Lipid BO) from pig lungs, and group-specific lipids A and O (Lipid A and Lipid O) were isolated from the former mixture by the aid of anti-A and anti-O sera and group-specific lipid B (Lipid B) from the latter by the aid of an anti-B serum.
2. Lipids AO, BO, A, O and B proved 'all electrophoretically homogeneous. The boundaries of the group-specific lipids (A, O and B) migrated with mobilities practically identical with those of their respective materials Lipids AO and BO.
3. Lipids A and O had the same solubility as Lipid AO, and Lipid B the same solubility as Lipid BO.
4. Test-tube tests resulted same among the substances.
5. The group-specific lipids A, O and B proved to be made up of the same components qualitatively as well as quantitatively as their respective materials (AO and BO) from which they had been separated, in so far as was examined.
6. These findings affirm our belief that those group-specific lipids are chemical entities but not mixtures of lipid and group mucopolysaccharide. In addition, the individual occurrence of substances A, B and O but not of substances AO and BO has been established concerning the group lipids of the present kind.
